A new reporter system for detection of retroviral infection

V. Planelles, A. Haislip, E. S. Withers-Ward, S. A. Stewart, Y. Xie, N. P. Shah, I. S.Y. Chen

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We describe a novel reporter molecule, the murine surface antigen Thy-1, useful for immunoselection and detection of retrovirus-mediated transduction by flow cytometry. A cDNA encoding the murine thy-1 gene was isolated, and cell surface expression of its gene product was demonstrated. The Thy-1 glycoprotein was tested as a cell surface reporter molecule in the context of replication-defective and -component retroviruses. Cells transduced via murine retroviral vectors carrying the thy-1 and the neomycin phosphotransferase genes express Thy-1 glycoprotein on their surfaces. The Thy-1 marker is potentially useful in gene transfer protocols because selection of transduced cells can be achieved by immunoselection with anti-Thy-1 antibodies shortly after infection with the retroviral vector. In addition, a human immunodeficiency virus type 1 (HIV-1) recombinant expressing thy-1 is described, which is replication-competent and syncytium-inducing in human peripheral blood mononuclear cells (PBMCs) and immortalized CD4-positive cell lines. Cells infected with this HIV-1 recombinant express Thy-1 on their surfaces and can be detected and purified by fluorescence-activated cell sorting (FACS). Because of these properties, retroviruses expressing this genetic marker can be useful for studies in gene therapy and of the retroviral life-cycle.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
JournalGene therapy
Volume2
Issue number6
StatePublished - Aug 24 1995
Externally publishedYes

Keywords

  • HIV-1
  • Thy-1
  • flow cytometry
  • gene therapy
  • retroviral vector
  • viral detection

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  • Cite this

    Planelles, V., Haislip, A., Withers-Ward, E. S., Stewart, S. A., Xie, Y., Shah, N. P., & Chen, I. S. Y. (1995). A new reporter system for detection of retroviral infection. Gene therapy, 2(6), 369-376.