A new class of cyclin dependent kinase in chlamydomonas is required for coupling cell size to cell division

Yubing Li, Dianyi Liu, Cristina López-Paz, Bradley J.S.C. Olson, James G. Umen

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Proliferating cells actively control their size by mechanisms that are poorly understood. The unicellular green alga Chlamydomonas reinhardtii divides by multiple fission, wherein a ‘counting’ mechanism couples mother cell-size to cell division number allowing production of uniform-sized daughters. We identified a sizer protein, CDKG1, that acts through the retinoblastoma (RB) tumor suppressor pathway as a D-cyclin-dependent RB kinase to regulate mitotic counting. Loss of CDKG1 leads to fewer mitotic divisions and large daughters, while mis-expression of CDKG1 causes supernumerous mitotic divisions and small daughters. The concentration of nuclear-localized CDKG1 in pre-mitotic cells is set by mother cell size, and its progressive dilution and degradation with each round of cell division may provide a link between mother cell-size and mitotic division number. Cell-size-dependent accumulation of limiting cell cycle regulators such as CDKG1 is a potentially general mechanism for size control.

Original languageEnglish
Article numbere10767
JournaleLife
Volume5
Issue numberMARCH2016
DOIs
StatePublished - Mar 25 2016

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