A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts

  • Krista K. Bowman
  • , Khalifah Sidik
  • , Colin A. Smith
  • , John stephen Taylor
  • , Paul W. Doetsch
  • , Greg A. Freyer

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S.pombe DNA endonuclease (SPDE) catalyzes a single ATPindependent incision immediately 5′ to the UV photoproduct and generates termini containing 3′ hydroxyl and 5′ phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/ cleavage step of a DNA excision repair pathway.

Original languageEnglish
Pages (from-to)3026-3032
Number of pages7
JournalNucleic acids research
Volume22
Issue number15
DOIs
StatePublished - Aug 11 1994

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