A multiscale model linking ion-channel molecular dynamics and electrostatics to the cardiac action potential

Jonathan R. Silva, Hua Pan, Dick Wu, Ali Nekouzadeh, Keith F. Decker, Jianmin Cui, Nathan A. Baker, David Sept, Yoram Rudy

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

Ion-channel function is determined by its gating movement. Yet, molecular dynamics and electrophysiological simulations were never combined to link molecular structure to function. We performed multiscale molecular dynamics and continuum electrostatics calculations to simulate a cardiac K+ channel (IKs) gating and its alteration by mutations that cause arrhythmias and sudden death. An all-atom model of the IKs α-subunit KCNQ1, based on the recent Kv1.2 structure, is used to calculate electrostatic energies during gating. Simulations are compared with experiments where varying degrees of positive charge-added via point mutation-progressively reduce current. Whole-cell simulations show that mutations cause action potential and ECG QT interval prolongation, consistent with clinical phenotypes. This framework allows integration of multiscale observations to study the molecular basis of excitation and its alteration by disease.

Original languageEnglish
Pages (from-to)11102-11106
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number27
DOIs
StatePublished - Jul 7 2009

Keywords

  • Cardiac arrhythmias
  • Long QT syndrome

Fingerprint

Dive into the research topics of 'A multiscale model linking ion-channel molecular dynamics and electrostatics to the cardiac action potential'. Together they form a unique fingerprint.

Cite this