@article{c374da587cd6468a928e8f0f8a625594,
title = "A multimodal cell census and atlas of the mammalian primary motor cortex",
abstract = "Here we report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties and cellular resolution input-output mapping, integrated through cross-modal computational analysis. Our results advance the collective knowledge and understanding of brain cell-type organization1-5. First, our study reveals a unified molecular genetic landscape of cortical cell types that integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a consensus taxonomy of transcriptomic types and their hierarchical organization that is conserved from mouse to marmoset and human. Third, in situ single-cell transcriptomics provides a spatially resolved cell-type atlas of the motor cortex. Fourth, cross-modal analysis provides compelling evidence for the transcriptomic, epigenomic and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types. We further present an extensive genetic toolset for targeting glutamatergic neuron types towards linking their molecular and developmental identity to their circuit function. Together, our results establish a unifying and mechanistic framework of neuronal cell-type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties.",
author = "{BRAIN Initiative Cell Census Network (BICCN)} and Callaway, {Edward M.} and Dong, {Hong Wei} and Ecker, {Joseph R.} and Hawrylycz, {Michael J.} and Huang, {Z. Josh} and Lein, {Ed S.} and John Ngai and Pavel Osten and Bing Ren and Tolias, {Andreas Savas} and Owen White and Hongkui Zeng and Xiaowei Zhuang and Ascoli, {Giorgio A.} and Behrens, {M. Margarita} and Jerold Chun and Guoping Feng and Gee, {James C.} and Ghosh, {Satrajit S.} and Ronna Hertzano and Lim, {Byung Kook} and Martone, {Maryann E.} and Lior Pachter and Ropelewski, {Alexander J.} and Tickle, {Timothy L.} and Yang, {X. William} and Bakken, {Trygve E.} and Philipp Berens and Daigle, {Tanya L.} and Jorstad, {Nikolas L.} and Kalmbach, {Brian E.} and Matho, {Katherine S.} and Mukamel, {Eran A.} and Maitham Naeemi and Federico Scala and Ting, {Jonathan T.} and Meng Zhang and Zhuzhu Zhang and Brian Zingg and Harris, {Julie A.} and Ethan Armand and Pengcheng Tan and Fangming Xie and Jingtian Zhou and Darren Bertagnolli and Tamara Casper and Kirsten Crichton and Nick Dee and Dinh Diep and Ding, {Song Lin} and Weixiu Dong and Dougherty, {Elizabeth L.} and Olivia Fong and Melissa Goldman and Jeff Goldy and Hodge, {Rebecca D.} and Lijuan Hu and Keene, {C. Dirk} and Krienen, {Fenna M.} and Matthew Kroll and Lake, {Blue B.} and Kanan Lathia and Sten Linnarsson and Macosko, {Evan Z.} and McCarroll, {Steven A.} and Delissa McMillen and Nadaf, {Naeem M.} and Nguyen, {Thuc Nghi} and Palmer, {Carter R.} and Thanh Pham and Nongluk Plongthongkum and Reed, {Nora M.} and Christine Rimorin and Romanow, {William J.} and Steven Savoia and Kimberly Siletti and Kimberly Smith and Josef Sulc and Bosiljka Tasic and Michael Tieu and Amy Torkelson and Herman Tung and {Van Velthoven}, {Cindy T.J.} and Vanderburg, {Charles R.} and Yanny, {Anna Marie} and Rongxin Fang and Xiaomeng Hou and Li, {Yang Eric} and Liu, {Christine S.} and Lucero, {Jacinta D.} and Osteen, {Julia K.} and Antonio Pinto-Duarte and Olivier Poirion and Sebastian Preissl and Xinxin Wang and Kun Zhang and Aldridge, {Andrew I.} and Lara Boggeman and Castanon, {Rosa G.} and Huaming Chen and Conor Fitzpatrick and Hanqing Liu and Chongyuan Luo and Michael Nunn and Rivkin, {Angeline C.} and Anna Bartlett and Carolyn O{\textquoteright}Connor and Bertha Dominguez and Tony Ito-Cole and Matthew Jacobs and Xin Jin and Lee, {Cheng Ta} and Lee, {Kuo Fen} and Miyazaki, {Paula Assakura} and Nery, {Joseph R.} and Yan Pang and Mohammad Rashid and Smith, {Jared B.} and Minh Vu and Elora Williams and Tommaso Biancalani and Booeshaghi, {A. Sina} and Megan Crow and Sandrine Dudoit and Stephan Fischer and Jesse Gillis and Qiwen Hu and Kharchenko, {Peter V.} and Niu, {Sheng Yong} and Vasilis Ntranos and Elizabeth Purdom and Aviv Regev and Davide Risso and {De Bezieux}, {Hector Roux} and Saroja Somasundaram and Kelly Street and Valentine Svensson and Wei Tian and Vaishnav, {Eeshit Dhaval} and {Van Den Berge}, Koen and Welch, {Joshua D.} and Bateup, {Helen S.} and Ian Bowman and Chance, {Rebecca K.} and Foster, {Nicholas N.} and William Galbavy and Hui Gong and Lin Gou and Hatfield, {Joshua T.} and Houri Hintiryan and Hirokawa, {Karla E.} and Gukhan Kim and Kramer, {Daniel J.} and Anan Li and Xiangning Li and Qingming Luo and Rodrigo Munoz-Castaneda and Lydia Ng and Stafford, {David A.} and Zhao Feng and Xueyan Jia and Shengdian Jiang and Tao Jiang and Xiuli Kuang and Rachael Larsen and Phil Lesnar and Yaoyao Li and Yuanyuan Li and Lijuan Liu and Hanchuan Peng and Lei Qu and Miao Ren and Zongcai Ruan and Elise Shen and Yuanyuan Song and Wayne Wakeman and Peng Wang and Yimin Wang and Lulu Yin and Jing Yuan and Sujun Zhao and Xuan Zhao and Arun Narasimhan and Ramesh Palaniswamy and Samik Banerjee and Liya Ding and Dhananjay Huilgol and Bingxing Huo and Kuo, {Hsien Chi} and Sophie Laturnus and Xu Li and Mitra, {Partha P.} and Judith Mizrachi and Quanxin Wang and Yun Wang and Peng Xie and Feng Xiong and Yang Yu and Eichhorn, {Stephen W.} and Shalaka Mulherkar",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2021.",
year = "2021",
doi = "10.1038/s41586-021-03950-0",
language = "English",
volume = "598",
pages = "86--102",
journal = "Nature",
issn = "0028-0836",
}