A mouse model for immunization with ex vivo virus-infected dendritic cells

C. B. López, A. Fernandez-Sesma, S. M. Czelusniak, J. L. Schulman, T. M. Moran

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Dendritic cells (DCs) have been demonstrated to be an important if not essential inducer of cellular immune responses. The ability to grow these cells in vitro may open up new avenues for protective immunizations. In this study we have analyzed the virus-specific memory response generated following immunization with ex vivo-infected bone marrow-derived dendritic cells. We demonstrate that mouse DCs are efficiently infected with influenza virus but do not release infectious progeny virus. Ex vivo-infected DCs secrete interleukin-12 (IL-12) and induce a potent T helper (Th)1-like immune response when injected into mice. This was demonstrated by the generation of cytotoxic T lymphocytes, the production of high levels of γ-interferon, and undetectable levels of IL-4 upon in vitro restimulation of splenocytes from immunized animals. In addition, the virus-specific antibody response is primarily of the IgG2a isotype, consistent with the expansion of Th1 cells. Animals immunized with DCs infected with X-31 influenza virus and challenged with PR8 influenza virus cleared the infection faster than animals not vaccinated. Thus, infected DCs efficiently activate the cellular immune response and induce heterosubtypic immunity in mice.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalCellular Immunology
Volume206
Issue number2
DOIs
StatePublished - Dec 15 2000

Keywords

  • Dendritic cells
  • Heterosubtypic immunity
  • Influenza virus
  • Mouse model

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