TY - JOUR
T1 - A Monoclonal Antibody-Based Enzyme Immunoassay for Detecting Parasite Antigenemia in Bancroftian Filariasis
AU - Weil, G. J.
AU - Jain, D. C.
AU - Santhanam, S.
AU - Malhotra, A.
AU - Kumar, H.
AU - Sethumadhavan, K. V.P.
AU - Liftis, F.
AU - Ghosh, T. K.
N1 - Funding Information:
Received for publication 30 October 1986, and in revised form 23 March 1987. This work was part of the INDO-US Science and Technology Initiative and was supported by grants AI-15353 and AI-22488 from the National Institute of Allergy and Infectious Diseases and by the Indian Council of Medical Research. We thank the staffs of the National Institute of Communicable Diseases in Delhi, Calicut, and Chetikade for their technical assistance. Please address requests for reprints to Dr. Gary J. Weil, The Jewish Hospital at Washington University Medical Center, 216 South Kingshighway, St. Louis, Missouri 63110.
PY - 1987/8
Y1 - 1987/8
N2 - We evaluated a monoclonal antibody-based enzyme immunoassay for detecting soluble parasite antigen in sera collected in an area in South India endemic for Wuchereria bancrofti. Filarial antigen was detected in sera from 56 of 57 microfilaremic patients, 9 of 64 amicrofilaremic patients with clinical filariasis, and 11 of 70 endemic controls. Antigen was not detected in sera from patients from nonendemic areas who had a variety of other filarial and nonfilarial helminth infections. Parasite antigen titers were significantly correlated with microfilarial counts in night blood smears (r =.64, P `.01). Negative antigen tests in patients with clinical filariasis may be explained in part by antibody-mediated clearance of circulating antigen. Antibodies to circulating W. bancrofti antigen were detected in 41 of 55 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W. bancrofti infection.
AB - We evaluated a monoclonal antibody-based enzyme immunoassay for detecting soluble parasite antigen in sera collected in an area in South India endemic for Wuchereria bancrofti. Filarial antigen was detected in sera from 56 of 57 microfilaremic patients, 9 of 64 amicrofilaremic patients with clinical filariasis, and 11 of 70 endemic controls. Antigen was not detected in sera from patients from nonendemic areas who had a variety of other filarial and nonfilarial helminth infections. Parasite antigen titers were significantly correlated with microfilarial counts in night blood smears (r =.64, P `.01). Negative antigen tests in patients with clinical filariasis may be explained in part by antibody-mediated clearance of circulating antigen. Antibodies to circulating W. bancrofti antigen were detected in 41 of 55 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W. bancrofti infection.
UR - http://www.scopus.com/inward/record.url?scp=0023192849&partnerID=8YFLogxK
U2 - 10.1093/infdis/156.2.350
DO - 10.1093/infdis/156.2.350
M3 - Article
C2 - 3298458
AN - SCOPUS:0023192849
SN - 0022-1899
VL - 156
SP - 350
EP - 355
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -