A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens

Yingjun Yan, Liwei Jiang, Karl J. Aufderheide, Gus A. Wright, Alexander Terekhov, Lino Costa, Kevin Qin, W. Tyler McCleery, John J. Fellenstein, Alessandro Ustione, J. Brian Robertson, Carl Hirschie Johnson, David W. Piston, M. Shane Hutson, John P. Wikswo, William Hofmeister, Chris Janetopoulos

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalMicroscopy and Microanalysis
Volume20
Issue number1
DOIs
StatePublished - Feb 2014

Keywords

  • Laser machining
  • Microcompression
  • Microfluidics
  • Paramecium tetraurelia
  • Polydimethylsiloxane
  • Pyramidal wells
  • Saccharomyces cerevisiae

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