A method of purification of partially methylated alditol acetates in the methylation analysis of glycoproteins and glycopeptides

Mark E. Lowe, Bo Nilsson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A method for methylation analysis of intact glycoproteins is described. Starting with intact glycoprotein, the oligosaccharides are methylated, hydrolyzed, reduced, and acetylated. The partially methylated alditol acetates are then separated from noncarbohydrate contaminants on a silica gel G column. Partially methylated hexitol acetates are eluted from the column with petroleum ether:ethyl acetate (1:1, v v) and partially methylated N-acetylhexosaminitol acetates are subsequently eluted with methanol. Analysis by gas-liquid chromatography/mass spectrometry of the partially methylated alditol acetates shows no interfering contaminants. This method circumvents the need to make pronase glycopeptides and avoids the pitfalls of other methylation procedures.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalAnalytical Biochemistry
Volume136
Issue number1
DOIs
StatePublished - Jan 1984
Externally publishedYes

Keywords

  • N-linked
  • glycopeptide
  • glycoprotein
  • methylation
  • oligosaccharide
  • silica

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