TY - JOUR
T1 - A method for preserving and visualizing the three-dimensional structure of the mouse zonule
AU - Bassnett, Steven
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/8
Y1 - 2019/8
N2 - Mechanical failure of the ciliary zonule characterizes several ocular and systemic diseases. The mouse has emerged as a useful model system to investigate the composition and structure/function relationships of the zonule. However, visualizing the organization of the diaphanous fibers that comprise the zonule is technically challenging because the fibers do not take up conventional histological stains and are disrupted easily during processing. Here, we describe a simple method for maintaining physiological pressure within the mouse eye during fixation, and a gel-embedding technique for stabilizing the zonular fibers during subsequent tissue processing and imaging steps. This approach facilitates quantitative measurements of fiber number and cross-sectional dimensions and will allow the effects of targeted disruption of zonule components to be assessed systematically.
AB - Mechanical failure of the ciliary zonule characterizes several ocular and systemic diseases. The mouse has emerged as a useful model system to investigate the composition and structure/function relationships of the zonule. However, visualizing the organization of the diaphanous fibers that comprise the zonule is technically challenging because the fibers do not take up conventional histological stains and are disrupted easily during processing. Here, we describe a simple method for maintaining physiological pressure within the mouse eye during fixation, and a gel-embedding technique for stabilizing the zonular fibers during subsequent tissue processing and imaging steps. This approach facilitates quantitative measurements of fiber number and cross-sectional dimensions and will allow the effects of targeted disruption of zonule components to be assessed systematically.
UR - http://www.scopus.com/inward/record.url?scp=85067274845&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2019.05.025
DO - 10.1016/j.exer.2019.05.025
M3 - Short survey
C2 - 31158380
AN - SCOPUS:85067274845
SN - 0014-4835
VL - 185
JO - Experimental eye research
JF - Experimental eye research
M1 - 107685
ER -