A method for isolating and analyzing human mRNA from newborn stool

William E. Bennett, Rosbel González-Rivera, Nurmohammad Shaikh, Vincent Magrini, Michelle Boykin, Barbara B. Warner, Aaron Hamvas, Phillip I. Tarr

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Efforts to characterize the human transcriptome have largely been limited to blood, urine, and tissue analyses (i.e., normally sterile materials). We report here an extraction protocol using commercially available reagents to obtain high-yield, reverse-transcribable RNA from human stool. Quantitative reverse transcriptase polymerase chain reactions demonstrated minimal intra-specimen but considerable intra-subject variability over time of transcripts for interleukin-6 (IL-6), IL-8, epidermal growth factor (EGF), calprotectin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This technique now expands opportunities to use the human fecal transcriptome to characterize gastrointestinal pathophysiology.

Original languageEnglish
Pages (from-to)56-60
Number of pages5
JournalJournal of Immunological Methods
Volume349
Issue number1-2
DOIs
StatePublished - Sep 30 2009

Keywords

  • Newborn
  • Nucleic acid purification
  • RNA
  • Stool
  • Transcriptome

Fingerprint Dive into the research topics of 'A method for isolating and analyzing human mRNA from newborn stool'. Together they form a unique fingerprint.

  • Cite this

    Bennett, W. E., González-Rivera, R., Shaikh, N., Magrini, V., Boykin, M., Warner, B. B., Hamvas, A., & Tarr, P. I. (2009). A method for isolating and analyzing human mRNA from newborn stool. Journal of Immunological Methods, 349(1-2), 56-60. https://doi.org/10.1016/j.jim.2009.07.013