A Map of the Interactome Network of the Metazoan C. elegans

Siming Li; Christopher M. Armstrong; Nicolas Bertin; Hui Ge; Stuart Milstein; Mike Boxem; Pierre Olivier Vidalain; Jing Dong J. Han; Alban Chesneau; Tong Hao; Debra S. Goldberg; Ning Li; Monica Martinez; Jean François Rual; Philippe Lamesch; Lai Xu; Muneesh Tewari; Sharyl L. Wong; Lan V. Zhang; Gabriel F. Berriz; Laurent Jacotot; Philippe Vaglio; Jérôme Reboul; Tomoko Hirozane-Kishikawa; Qianru Li; Harrison W. Gabel; Ahmed Elewa; Bridget Baumgartner; Debra J. Rose; Haiyuan Yu; Stephanie Bosak; Reynaldo Sequerra; Andrew Fraser; Susan E. Mango; William M. Saxton; Susan Strome; Sander v. Van Den Heuvel; Fabio Piano; Jean Vandenhaute; Claude Sardet; Mark Gerstein; Lynn Doucette-Stamm; Kristin C. Gunsalus; J. Wade Harper; Michael E. Cusick; Frederick P. Roth; David E. Hill; Marc Vidal

doi:10.1126/science.1091403

A Map of the Interactome Network of the Metazoan C. elegans

Siming Li, Christopher M. Armstrong, Nicolas Bertin, Hui Ge, Stuart Milstein, Mike Boxem, Pierre Olivier Vidalain, Jing Dong J. Han, Alban Chesneau, Tong Hao, Debra S. Goldberg, Ning Li, Monica Martinez, Jean François Rual, Philippe Lamesch, Lai Xu, Muneesh Tewari, Sharyl L. Wong, Lan V. Zhang, Gabriel F. BerrizLaurent Jacotot, Philippe Vaglio, Jérôme Reboul, Tomoko Hirozane-Kishikawa, Qianru Li, Harrison W. Gabel, Ahmed Elewa, Bridget Baumgartner, Debra J. Rose, Haiyuan Yu, Stephanie Bosak, Reynaldo Sequerra, Andrew Fraser, Susan E. Mango, William M. Saxton, Susan Strome, Sander v. Van Den Heuvel, Fabio Piano, Jean Vandenhaute, Claude Sardet, Mark Gerstein, Lynn Doucette-Stamm, Kristin C. Gunsalus, J. Wade Harper, Michael E. Cusick, Frederick P. Roth, David E. Hill, Marc Vidal

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Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

Original language	English
Pages (from-to)	540-543
Number of pages	4
Journal	Science
Volume	303
Issue number	5657
DOIs	https://doi.org/10.1126/science.1091403
State	Published - Jan 23 2004

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Li, S., Armstrong, C. M., Bertin, N., Ge, H., Milstein, S., Boxem, M., Vidalain, P. O., Han, J. D. J., Chesneau, A., Hao, T., Goldberg, D. S., Li, N., Martinez, M., Rual, J. F., Lamesch, P., Xu, L., Tewari, M., Wong, S. L., Zhang, L. V., ... Vidal, M. (2004). A Map of the Interactome Network of the Metazoan C. elegans. Science, 303(5657), 540-543. https://doi.org/10.1126/science.1091403

@article{9ab582f545a14273b0a2f6d2a28cc5fd,

title = "A Map of the Interactome Network of the Metazoan C. elegans",

abstract = "To initiate studies on how protein-protein interaction (or {"}interactome{"}) networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.",

author = "Siming Li and Armstrong, {Christopher M.} and Nicolas Bertin and Hui Ge and Stuart Milstein and Mike Boxem and Vidalain, {Pierre Olivier} and Han, {Jing Dong J.} and Alban Chesneau and Tong Hao and Goldberg, {Debra S.} and Ning Li and Monica Martinez and Rual, {Jean Fran{\c c}ois} and Philippe Lamesch and Lai Xu and Muneesh Tewari and Wong, {Sharyl L.} and Zhang, {Lan V.} and Berriz, {Gabriel F.} and Laurent Jacotot and Philippe Vaglio and J{\'e}r{\^o}me Reboul and Tomoko Hirozane-Kishikawa and Qianru Li and Gabel, {Harrison W.} and Ahmed Elewa and Bridget Baumgartner and Rose, {Debra J.} and Haiyuan Yu and Stephanie Bosak and Reynaldo Sequerra and Andrew Fraser and Mango, {Susan E.} and Saxton, {William M.} and Susan Strome and {Van Den Heuvel}, {Sander v.} and Fabio Piano and Jean Vandenhaute and Claude Sardet and Mark Gerstein and Lynn Doucette-Stamm and Gunsalus, {Kristin C.} and Harper, {J. Wade} and Cusick, {Michael E.} and Roth, {Frederick P.} and Hill, {David E.} and Marc Vidal",

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Li, S, Armstrong, CM, Bertin, N, Ge, H, Milstein, S, Boxem, M, Vidalain, PO, Han, JDJ, Chesneau, A, Hao, T, Goldberg, DS, Li, N, Martinez, M, Rual, JF, Lamesch, P, Xu, L, Tewari, M, Wong, SL, Zhang, LV, Berriz, GF, Jacotot, L, Vaglio, P, Reboul, J, Hirozane-Kishikawa, T, Li, Q, Gabel, HW, Elewa, A, Baumgartner, B, Rose, DJ, Yu, H, Bosak, S, Sequerra, R, Fraser, A, Mango, SE, Saxton, WM, Strome, S, Van Den Heuvel, SV, Piano, F, Vandenhaute, J, Sardet, C, Gerstein, M, Doucette-Stamm, L, Gunsalus, KC, Harper, JW, Cusick, ME, Roth, FP, Hill, DE & Vidal, M 2004, 'A Map of the Interactome Network of the Metazoan C. elegans', Science, vol. 303, no. 5657, pp. 540-543. https://doi.org/10.1126/science.1091403

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T1 - A Map of the Interactome Network of the Metazoan C. elegans

AU - Li, Siming

AU - Armstrong, Christopher M.

AU - Bertin, Nicolas

AU - Ge, Hui

AU - Milstein, Stuart

AU - Boxem, Mike

AU - Vidalain, Pierre Olivier

AU - Han, Jing Dong J.

AU - Chesneau, Alban

AU - Hao, Tong

AU - Goldberg, Debra S.

AU - Li, Ning

AU - Martinez, Monica

AU - Rual, Jean François

AU - Lamesch, Philippe

AU - Xu, Lai

AU - Tewari, Muneesh

AU - Wong, Sharyl L.

AU - Zhang, Lan V.

AU - Berriz, Gabriel F.

AU - Jacotot, Laurent

AU - Vaglio, Philippe

AU - Reboul, Jérôme

AU - Hirozane-Kishikawa, Tomoko

AU - Li, Qianru

AU - Gabel, Harrison W.

AU - Elewa, Ahmed

AU - Baumgartner, Bridget

AU - Rose, Debra J.

AU - Yu, Haiyuan

AU - Bosak, Stephanie

AU - Sequerra, Reynaldo

AU - Fraser, Andrew

AU - Mango, Susan E.

AU - Saxton, William M.

AU - Strome, Susan

AU - Van Den Heuvel, Sander v.

AU - Piano, Fabio

AU - Vandenhaute, Jean

AU - Sardet, Claude

AU - Gerstein, Mark

AU - Doucette-Stamm, Lynn

AU - Gunsalus, Kristin C.

AU - Harper, J. Wade

AU - Cusick, Michael E.

AU - Roth, Frederick P.

AU - Hill, David E.

AU - Vidal, Marc

PY - 2004/1/23

Y1 - 2004/1/23

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AB - To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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U2 - 10.1126/science.1091403

DO - 10.1126/science.1091403

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AN - SCOPUS:9144264169

SN - 0036-8075

VL - 303

SP - 540

EP - 543

JO - Science

JF - Science

IS - 5657

ER -

A Map of the Interactome Network of the Metazoan C. elegans

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