A liquid-like organelle at the root of motile ciliopathy

Ryan L. Huizar, Chanjae Lee, Alexander A. Boulgakov, Amjad Horani, Fan Tu, Edward M. Marcotte, Steven L. Brody, John B. Wallingford

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Motile ciliopathies are characterized by specific defects in cilia beating that result in chronic airway disease, subfertility, ectopic pregnancy, and hydrocephalus. While many patients harbor mutations in the dynein motors that drive cilia beating, the disease also results from mutations in so-called Dynein Axonemal Assembly Factors (DNAAFs) that act in the cytoplasm. The mechanisms of DNAAF action remain poorly defined. Here, we show that DNAAFs concentrate together with axonemal dyneins and chaperones into organelles that form specifically in multiciliated cells, which we term DynAPs, for Dynein Axonemal Particles. These organelles display hallmarks of biomolecular condensates, and remarkably, DynAPs are enriched for the stress granule protein G3bp1, but not for other stress granule proteins or P-body proteins. Finally, we show that both the formation and the liquid-like behaviors of DynAPs are disrupted in a model of motile ciliopathy.

Original languageEnglish
Article numbere38497
JournaleLife
Volume7
DOIs
StatePublished - Dec 1 2018

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