TY - JOUR
T1 - A latent, nonpathogenic HSV-1-derived vector stably expresses β-galactosidase in mouse neurons
AU - Dobson, Anthony T.
AU - Margolis, Todd P.
AU - Sedarati, Farhad
AU - Stevens, Jack G.
AU - Feldman, Lawrence T.
N1 - Funding Information:
We thank C. Cepko for kindly providing the BAG vector, R. Sandri-Goldin for plasmid pSG28, K. Izumi for the LAT-negative virus KOS 8117, and N. DeLuca for the ICP4-expressing cell line E5. This work was supported by Public Health Service grants AI28338 to L. T. F. and AI06246 to J. G. S. from the National Institutes of Health. A. T. D. was supported by Medical Scientist training grant program grant GM08042. Technical support was provided by Vivian Dissette. T. P. M. was supported by a National Research Service Award EY06190 from the National Eye Institute and by a Heed/Knapp fellowship. F. S. was supported by postdoctoral fellowship FG-776-A-1 from the National Multiple Sclerosis Society. L. T. F. is a recipient of a faculty research award FRA-340 from the American Cancer Society. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1990/9
Y1 - 1990/9
N2 - A genetically engineered herpes simplex virus variant was constructed for use as a stable gene vector for neurons. To inhibit replication, the agent possessed a deletion in the immediate early gene ICP4, and to minimize reactivation from the latent state, the gene encoding the latency-associated transcript was deleted. The E. coli β-galactosidase gene under the control of the Maloney murine leukemia virus long terminal repeat promoter was inserted into the ICP4 region. When introduced into the peripheral nervous system, this virus established latent infections and stably expressed β-galactosidase in primary sensory neurons. Expression of β-galactosidase over a more limited time period was observed when the latent infection was established in motor neurons of the hypoglossal nucleus. Agents of this general design have considerable potential for use as gene vectors for studies of neuronal function and correction of genetic defects affecting neurons.
AB - A genetically engineered herpes simplex virus variant was constructed for use as a stable gene vector for neurons. To inhibit replication, the agent possessed a deletion in the immediate early gene ICP4, and to minimize reactivation from the latent state, the gene encoding the latency-associated transcript was deleted. The E. coli β-galactosidase gene under the control of the Maloney murine leukemia virus long terminal repeat promoter was inserted into the ICP4 region. When introduced into the peripheral nervous system, this virus established latent infections and stably expressed β-galactosidase in primary sensory neurons. Expression of β-galactosidase over a more limited time period was observed when the latent infection was established in motor neurons of the hypoglossal nucleus. Agents of this general design have considerable potential for use as gene vectors for studies of neuronal function and correction of genetic defects affecting neurons.
UR - http://www.scopus.com/inward/record.url?scp=0025031673&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(90)90171-B
DO - 10.1016/0896-6273(90)90171-B
M3 - Article
C2 - 2169271
AN - SCOPUS:0025031673
SN - 0896-6273
VL - 5
SP - 353
EP - 360
JO - Neuron
JF - Neuron
IS - 3
ER -