A human forkhead/winged-helix transcription factor expressed in developing pulmonary and renal epithelium

Glenn J. Pelletier, Steven L. Brody, Helen Liapis, Robert A. White, Brian P. Hackett

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Members of the forkhead/winged-helix transcription factor family play crucial roles during vertebrate development. A human hepatocyte nuclear factor/forkhead homolog (HFH)-4 cDNA encoding a 421-amino acid protein was isolated from a human fetal lung cDNA library. By Southern blot analysis of human-rodent somatic cell hybrid genomic DNA, the human HFH-4 gene localizes to chromosome 17q23-qter. This is the locus of another forkhead/winged-helix gene, the interleukin enhancer binding factor gene. RNA blot analysis revealed a 2.5-kilobase human HFH-4 transcript in fetal lung, kidney, and brain as well as in adult reproductive tissues, lung, and brain. By in situ hybridization, HFH-4 expression is associated with differentiation of the proximal pulmonary epithelium, starting during the pseudoglandular stage of human lung development. During human renal morphogenesis, HFH-4 is expressed in the developing epithelial cells of the ureteric duct, glomerulus, and epithelial vesicles. The unique pattern of HFH-4 expression during human fetal development suggests a role for this forkhead/winged-helix factor during pulmonary and renal epithelial development.

Original languageEnglish
Pages (from-to)L351-L359
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume274
Issue number3 18-3
DOIs
StatePublished - Mar 1998

Keywords

  • Gene expression
  • Human development
  • Transcription factors

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