TY - JOUR
T1 - A hepatic reticuloendothelial cell receptor specific for SO4-4GalNAcβ1, 4GlcNAcβ1,2Manα that mediates rapid clearance of lutropin
AU - Fiete, Dorothy
AU - Srivastava, Vandana
AU - Hindsgaul, Ole
AU - Baenziger, Jacques U.
N1 - Funding Information:
This work was supported by R37-CA-21923, ROl-DK-41738, and a Monsanto-Washington University Biomedical Research Grant. We thank Paul H. Weigel for his gift of the Bolton-Hunter-hyaluronate-hexylamine adduct.
PY - 1991/12/20
Y1 - 1991/12/20
N2 - We have identified a receptor in hepatic endothelial and Kupffer cells that binds oligosaccharides terminating with the sequence SO4-4GalNAcβ1,4GlcNAcβ1,2-Manα (S4GGnM). This receptor can account for the rapid removal of the glycoprotein hormone lutropin, which bears unique Asn-linked oligosaccharides terminating in S4GGnM, from the circulation. Hepatic endothelial cells express 579,000 S4GGnM receptors at their surface and bind lutropin with an apparent Kd of 1.63 × 10-7 M. Bound ligand is rapidly internalized. Binding does not require divalent cations, is reversed by incubation at pH 5.0 or below, and is inhibited by fucoidin but not by hyaluronate, heparin, chondroitin sulfate, or dextran sulfate. We propose that the S4GGnM-specific receptor represents a major mechanism for clearance of certain sulfated glycoproteins from the blood, including members of the glycoprotein hormone family.
AB - We have identified a receptor in hepatic endothelial and Kupffer cells that binds oligosaccharides terminating with the sequence SO4-4GalNAcβ1,4GlcNAcβ1,2-Manα (S4GGnM). This receptor can account for the rapid removal of the glycoprotein hormone lutropin, which bears unique Asn-linked oligosaccharides terminating in S4GGnM, from the circulation. Hepatic endothelial cells express 579,000 S4GGnM receptors at their surface and bind lutropin with an apparent Kd of 1.63 × 10-7 M. Bound ligand is rapidly internalized. Binding does not require divalent cations, is reversed by incubation at pH 5.0 or below, and is inhibited by fucoidin but not by hyaluronate, heparin, chondroitin sulfate, or dextran sulfate. We propose that the S4GGnM-specific receptor represents a major mechanism for clearance of certain sulfated glycoproteins from the blood, including members of the glycoprotein hormone family.
UR - http://www.scopus.com/inward/record.url?scp=0026314891&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(91)90287-9
DO - 10.1016/0092-8674(91)90287-9
M3 - Article
C2 - 1662117
AN - SCOPUS:0026314891
SN - 0092-8674
VL - 67
SP - 1103
EP - 1110
JO - Cell
JF - Cell
IS - 6
ER -