A Gorilla Adenovirus-Based Vaccine against Zika Virus Induces Durable Immunity and Confers Protection in Pregnancy

Ahmed O. Hassan, Igor P. Dmitriev, Elena A. Kashentseva, Haiyan Zhao, Douglas E. Brough, Daved H. Fremont, David T. Curiel, Michael S. Diamond

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The teratogenic potential of Zika virus (ZIKV) has made the development of an effective vaccine a global health priority. Here, we generate two gorilla adenovirus-based ZIKV vaccines that encode for pre-membrane (prM) and envelope (E) proteins (GAd-Zvp) or prM and the ectodomain of E protein (GAd-Eecto). Both vaccines induce humoral and cell-mediated immune responses and prevent lethality after ZIKV challenge in mice. Protection is antibody dependent, CD8+ T cell independent, and for GAd-Eecto requires the complement component C1q. Immunization of GAd-Zvp induces antibodies against a key neutralizing epitope on domain III of E protein and confers durable protection as evidenced by memory B and long-lived plasma cell responses and challenge studies 9 months later. In two models of ZIKV infection during pregnancy, GAd-Zvp prevents maternal-to-fetal transmission. The gorilla adenovirus-based vaccine platform encoding full-length prM and E genes is a promising candidate for preventing congenital ZIKV syndrome and possibly infection by other flaviviruses.

Original languageEnglish
Pages (from-to)2634-2646.e4
JournalCell Reports
Volume28
Issue number10
DOIs
StatePublished - Sep 3 2019

Keywords

  • Zika virus
  • adaptive immunity
  • flavivirus
  • pathogenesis
  • pregnancy
  • vaccine

Fingerprint Dive into the research topics of 'A Gorilla Adenovirus-Based Vaccine against Zika Virus Induces Durable Immunity and Confers Protection in Pregnancy'. Together they form a unique fingerprint.

  • Cite this