TY - GEN
T1 - A GLASS MICROFLUIDIC BIOELECTROCHEMICAL CELL PLATFORM FOR THE STUDY OF MICROBIAL EXTRACELLULAR ELECTRON UPTAKE
AU - Stoica, Andreea
AU - Rengasamy, Karthikeyan
AU - Ranaivoarisoa, Tahina O.
AU - Bose, Arpita
AU - Meacham, J. Mark
N1 - Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Miniaturization of measurement systems provides several advantages including improved flexibility, decreased sample size, better sensitivity, and the opportunity to multiplex different measurement modalities, opening pathways to new experimental studies. We introduce a scalable glass-based microfluidic bioelectrochemical cell (μ-BEC) platform that miniaturizes a three-electrode electrochemical reactor and multiplexes electrochemical measurements while incorporating imaging capability and microfluidic control. We developed the platform for the study of extracellular electron uptake (EEU) in microbes and used Rhodopseudomonas palustris TIE-1 (TIE-1) bacteria as a model organism. We demonstrate the use of microfluidic flow to remove planktonic cells and isolate the EEU response of the biofilm.
AB - Miniaturization of measurement systems provides several advantages including improved flexibility, decreased sample size, better sensitivity, and the opportunity to multiplex different measurement modalities, opening pathways to new experimental studies. We introduce a scalable glass-based microfluidic bioelectrochemical cell (μ-BEC) platform that miniaturizes a three-electrode electrochemical reactor and multiplexes electrochemical measurements while incorporating imaging capability and microfluidic control. We developed the platform for the study of extracellular electron uptake (EEU) in microbes and used Rhodopseudomonas palustris TIE-1 (TIE-1) bacteria as a model organism. We demonstrate the use of microfluidic flow to remove planktonic cells and isolate the EEU response of the biofilm.
KW - Extracellular electron uptake
KW - Glass microfluidics
KW - Microfluidic bioelectrochemical cell
KW - Rhodopseudomonas palustris TIE-1
KW - Scalable microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85175733552&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85175733552
T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1179
EP - 1180
BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Y2 - 23 October 2022 through 27 October 2022
ER -