A genome-wide small interfering RNA screen identifies host factors required for vesicular stomatitis virus infection

Amy Si Ying Lee, Rebeca Burdeinick-Kerr, Sean P.J. Whelan

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Viruses are dependent on their host cells for replication and thus have evolved in intimate association with them. The identification of host factors required for viral infection has led to advances in both viral and cellular biology. Vesicular stomatitis virus (VSV), a negative-sense RNA virus, replicates in all eukaryotic cells in culture, suggesting that the host requirements for its replication are ubiquitous. In this study, we performed a genome-wide small interfering RNA screen of human cells in culture and identified multiple cellular genes that influence the entry and replication of VSV. From a list of > 300 genes, we selected the most promising candidates to perform further analysis to assign their functions to either the entry or intracellular replication step of infection. We implicate 3 new factors in VSV entry and 20 new factors in viral gene expression. These proteins have diverse cellular roles, including S-adenosylmethionine synthesis, respiration, and host translation machinery, underscoring the intimate relationship between VSV and the host cell. Together, these results provide a curated list of genes required for VSV replication.

Original languageEnglish
Pages (from-to)8355-8360
Number of pages6
JournalJournal of virology
Volume88
Issue number15
DOIs
StatePublished - 2014

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