TY - JOUR
T1 - A genome-scale metabolic model of anabaena 33047 to guide genetic modifications to overproduce nylon monomers
AU - Hendry, John I.
AU - Dinh, Hoang V.
AU - Sarkar, Debolina
AU - Wang, Lin
AU - Bandyopadhyay, Anindita
AU - Pakrasi, Himadri B.
AU - Maranas, Costas D.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - Nitrogen fixing-cyanobacteria can significantly improve the economic feasibility of cyanobac-terial production processes by eliminating the requirement for reduced nitrogen. Anabaena sp. ATCC 33047 is a marine, heterocyst forming, nitrogen fixing cyanobacteria with a very short doubling time of 3.8 h. We developed a comprehensive genome-scale metabolic (GSM) model, iAnC892, for this organism using annotations and content obtained from multiple databases. iAnC892 describes both the vegetative and heterocyst cell types found in the filaments of Anabaena sp. ATCC 33047. iAnC892 includes 953 unique reactions and accounts for the annotation of 892 genes. Comparison of iAnC892 reaction content with the GSM of Anabaena sp. PCC 7120 revealed that there are 109 reactions including uptake hydrogenase, pyruvate decarboxylase, and pyruvate-formate lyase unique to iAnC892. iAnC892 enabled the analysis of energy production pathways in the heterocyst by allowing the cell specific deactivation of light dependent electron transport chain and glucose-6-phosphate metabolizing pathways. The analysis revealed the importance of light dependent electron transport in generating ATP and NADPH at the required ratio for optimal N2 fixation. When used alongside the strain design algorithm, OptForce, iAnC892 recapitulated several of the experimentally successful genetic intervention strategies that over produced valerolactam and caprolactam precursors.
AB - Nitrogen fixing-cyanobacteria can significantly improve the economic feasibility of cyanobac-terial production processes by eliminating the requirement for reduced nitrogen. Anabaena sp. ATCC 33047 is a marine, heterocyst forming, nitrogen fixing cyanobacteria with a very short doubling time of 3.8 h. We developed a comprehensive genome-scale metabolic (GSM) model, iAnC892, for this organism using annotations and content obtained from multiple databases. iAnC892 describes both the vegetative and heterocyst cell types found in the filaments of Anabaena sp. ATCC 33047. iAnC892 includes 953 unique reactions and accounts for the annotation of 892 genes. Comparison of iAnC892 reaction content with the GSM of Anabaena sp. PCC 7120 revealed that there are 109 reactions including uptake hydrogenase, pyruvate decarboxylase, and pyruvate-formate lyase unique to iAnC892. iAnC892 enabled the analysis of energy production pathways in the heterocyst by allowing the cell specific deactivation of light dependent electron transport chain and glucose-6-phosphate metabolizing pathways. The analysis revealed the importance of light dependent electron transport in generating ATP and NADPH at the required ratio for optimal N2 fixation. When used alongside the strain design algorithm, OptForce, iAnC892 recapitulated several of the experimentally successful genetic intervention strategies that over produced valerolactam and caprolactam precursors.
KW - Anabaena sp. ATCC 33047
KW - Caprolactam
KW - Cyanobacteria
KW - Flux balance analysis
KW - Genome scale metabolic models
KW - Heterocyst
KW - OptForce
KW - Valerolactam
UR - http://www.scopus.com/inward/record.url?scp=85103115492&partnerID=8YFLogxK
U2 - 10.3390/metabo11030168
DO - 10.3390/metabo11030168
M3 - Article
C2 - 33804103
AN - SCOPUS:85103115492
SN - 2218-1989
VL - 11
JO - Metabolites
JF - Metabolites
IS - 3
M1 - 168
ER -