TY - JOUR
T1 - A genetic analysis of neural progenitor differentiation
AU - Geschwind, Daniel H.
AU - Ou, Jing
AU - Easterday, Mathew C.
AU - Dougherty, Joseph D.
AU - Jackson, Robert L.
AU - Chen, Zugen
AU - Antoine, Heath
AU - Terskikh, Alexey
AU - Weissman, Irving L.
AU - Nelson, Stanley F.
AU - Kornblum, Harley I.
N1 - Funding Information:
The authors thank M. Aldimassi and the UCLA DNA microarray facility for microarray fabrication, Dr. Evan Snyder for the gift of C17-2 cells, and the Mental Retardation Research Center Media Core for help with figures (Carol Gray). We acknowledge support from the National Institutes of Health (NIH), the National Institute of Mental Health (NIMH) grant MH60233 (D. H. G.), NIMH grant MH062800 (H. I. K. and D. H. G.), National Institute of Neurological Disorders and Stroke (NINDS) grant NS28383 (H. I. K.), and a National Institute of Child Health and Human Development (NICHD)/UCLA Child Health Research Center Award P30HD34610 (H. I. K.) and thank the Ron Shapiro Charitable Foundation for their generous support (D. H. G. and H. I. K.). M. C. E. is supported by the Training Program in Neural Repair (NS07449), J. D. D. is supported by a Howard Hughes Medical Institute Graduate Fellowship, and R. L. J. is supported by the NINDS training program in Neurorehabilitation (NS07479-08).
PY - 2001
Y1 - 2001
N2 - Genetic mechanisms regulating CNS progenitor function and differentiation are not well understood. We have used microarrays derived from a representational difference analysis (RDA) subtraction in a heterogeneous stem cell culture system to systematically study the gene expression patterns of CNS progenitors. This analysis identified both known and novel genes enriched in progenitor cultures. In situ hybridization in a subset of clones demonstrated that many of these genes were expressed preferentially in germinal zones, some showing distinct ventricular or subventricular zone labeling. Several genes were also enriched in hematopoietic stem cells, suggesting an overlap of gene expression in neural and hematopoietic progenitors. This combination of methods demonstrates the power of using custom microarrays derived from RDA-subtracted libraries for both gene discovery and gene expression analysis in the central nervous system.
AB - Genetic mechanisms regulating CNS progenitor function and differentiation are not well understood. We have used microarrays derived from a representational difference analysis (RDA) subtraction in a heterogeneous stem cell culture system to systematically study the gene expression patterns of CNS progenitors. This analysis identified both known and novel genes enriched in progenitor cultures. In situ hybridization in a subset of clones demonstrated that many of these genes were expressed preferentially in germinal zones, some showing distinct ventricular or subventricular zone labeling. Several genes were also enriched in hematopoietic stem cells, suggesting an overlap of gene expression in neural and hematopoietic progenitors. This combination of methods demonstrates the power of using custom microarrays derived from RDA-subtracted libraries for both gene discovery and gene expression analysis in the central nervous system.
UR - http://www.scopus.com/inward/record.url?scp=17744370294&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(01)00209-4
DO - 10.1016/S0896-6273(01)00209-4
M3 - Review article
C2 - 11239426
AN - SCOPUS:17744370294
SN - 0896-6273
VL - 29
SP - 325
EP - 339
JO - Neuron
JF - Neuron
IS - 2
ER -