@inbook{69ef7c9dee794915a64e3bd518047ea2,
title = "A functional assay to assess connexin 43-mediated cell-to-cell communication of second messengers in cultured bone cells",
abstract = "Cell-to-cell transfer of small molecules is a fundamental way by which multicellular organisms coordinate function. Recent work has highlighted the complexity of biologic responses downstream of gap junctions. As the connexin-regulated effectors are coming into focus, there is a need to develop functional assays that allow specific testing of biologically relevant second messengers. Here, we describe a modification of the classic gap junction parachute assay to assess biologically relevant molecules passed through gap junctions.",
keywords = "Cell-to-cell communication, Connexin 43, Gap junctions, Luciferase reporter assay, Osteoblast, Parachute assay, Transient transfection",
author = "Stains, {Joseph P.} and Roberto Civitelli",
note = "Funding Information: This work was supported by an NIH grant AR036361 to JPS and NIH grant AR041255 to RC. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3664-9_14",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "193--201",
booktitle = "Methods in Molecular Biology",
}