TY - JOUR
T1 - A Flexible, Quantitative Plasmonic-Fluor Lateral Flow Assay for the Rapid Detection of Orthoebolavirus zairense and Orthoebolavirus sudanense
AU - Qavi, Abraham J.
AU - Jiang, Qisheng
AU - Aman, M. Javad
AU - Vu, Hong
AU - Zetlin, Larry
AU - Dye, John M.
AU - Froude, Jeffrey W.
AU - Leung, Daisy W.
AU - Holtsberg, Frederick
AU - Crick, Scott L.
AU - Amarasinghe, Gaya K.
N1 - Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
PY - 2024/1/12
Y1 - 2024/1/12
N2 - Filoviruses comprise a family of single-stranded, negative-sense RNA viruses with a significant impact on human health. Given the risk for disease outbreaks, as highlighted by the recent outbreaks across Africa, there is an unmet need for flexible diagnostic technologies that can be deployed in resource-limited settings. Herein, we highlight the use of plasmonic-fluor lateral flow assays (PF-LFA) for the rapid, quantitative detection of an Ebolavirus-secreted glycoprotein, a marker for infection. Plasmonic fluors are a class of ultrabright reporter molecules that combine engineered nanorods with conventional fluorophores, resulting in improved analytical sensitivity. We have developed a PF-LFA for Orthoebolavirus zairense (EBOV) and Orthoebolavirus sudanense (SUDV) that provides estimated limits of detection as low as 0.446 and 0.641 ng/mL, respectively. Furthermore, our assay highlights a high degree of specificity between the two viral species while also maintaining a turnaround time as short as 30 min. To highlight the utility of our PF-LFA, we demonstrate the detection of EBOV infection in non-human primates. Our PF-LFA represents an enormous step forward in the development of a robust, field-deployable assay for filoviruses.
AB - Filoviruses comprise a family of single-stranded, negative-sense RNA viruses with a significant impact on human health. Given the risk for disease outbreaks, as highlighted by the recent outbreaks across Africa, there is an unmet need for flexible diagnostic technologies that can be deployed in resource-limited settings. Herein, we highlight the use of plasmonic-fluor lateral flow assays (PF-LFA) for the rapid, quantitative detection of an Ebolavirus-secreted glycoprotein, a marker for infection. Plasmonic fluors are a class of ultrabright reporter molecules that combine engineered nanorods with conventional fluorophores, resulting in improved analytical sensitivity. We have developed a PF-LFA for Orthoebolavirus zairense (EBOV) and Orthoebolavirus sudanense (SUDV) that provides estimated limits of detection as low as 0.446 and 0.641 ng/mL, respectively. Furthermore, our assay highlights a high degree of specificity between the two viral species while also maintaining a turnaround time as short as 30 min. To highlight the utility of our PF-LFA, we demonstrate the detection of EBOV infection in non-human primates. Our PF-LFA represents an enormous step forward in the development of a robust, field-deployable assay for filoviruses.
KW - Filoviruses
KW - diagnostics
KW - lateral flow assay
KW - plasmonics
UR - http://www.scopus.com/inward/record.url?scp=85180093772&partnerID=8YFLogxK
U2 - 10.1021/acsinfecdis.3c00423
DO - 10.1021/acsinfecdis.3c00423
M3 - Article
C2 - 38048277
AN - SCOPUS:85180093772
SN - 2373-8227
VL - 10
SP - 57
EP - 63
JO - ACS Infectious Diseases
JF - ACS Infectious Diseases
IS - 1
ER -