The interaction between receptor and a heterotrimeric G protein (αβγ) is thought to involve the intracellular loops of receptors and specific domains on the G protein. Here we show that a chemically farnesylated peptide (P5far) specific to the carboxyl-terminal domain (amino acids 60-71: DKNPFKELKGGC) of the γ subunit of the G protein, G(t), directly stabilizes the active form of rhodopsin, metarhodopsin II (M II), and also uncouples rhodopsin-G(t) interaction. Peptide activity is significantly affected by the absence of the isoprenoid moiety. Moreover, we show that altering the amino acid sequence of the farnesylated peptide by randomizing the sequence, substituting hydrophobic with hydrophilic residues (F64T; L67S) or deleting amino acids 60-66 significantly reduces the ability of the peptide to stabilize M II. This indicates that both the farnesyl moiety and the structure of the γ subunit tail are specific determinants of receptor-G protein interaction. These results also suggest a general function for the family of G protein γ subunits in signaling.
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|State||Published - Aug 26 1994|