A family of fluoride-specific ion channels with dual-topology architecture

Randy B. Stockbridge, Janice L. Robertson, Ludmila Kolmakova-Partensky, Christopher Miller

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Fluoride ion, ubiquitous in soil, water, and marine environments, is a chronic threat to microorganisms. Many prokaryotes, archea, unicellular eukaryotes, and plants use a recently discovered family of F- exporter proteins to lower cytoplasmic F- levels to counteract the anion's toxicity. We show here that these 'Fluc' proteins, purified and reconstituted in liposomes and planar phospholipid bilayers, form constitutively open anion channels with extreme selectivity for F- over Cl-. The active channel is a dimer of identical or homologous subunits arranged in anti parallel transmembrane orientation. This dual-topology assembly has not previously been seen in ion channels but is known in multi drug transporters of the SMR family, and is suggestive of an evolutionary antecedent of the inverted repeats found within the subunits of many membrane transport proteins.

Original languageEnglish
Article numbere01084
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Aug 27 2013
Externally publishedYes

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