A drought-responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome

Dhananjay Gotarkar, Toshisangba Longkumer, Naoki Yamamoto, Amrit Kaur Nanda, Tamara Iglesias, Lin Feng Li, Berta Miro, Elisa Blanco Gonzalez, Maria Montes Bayon, Kenneth M. Olsen, Yue Ie Caroline Hsing, Ajay Kohli

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Drought stress in plants causes differential expression of numerous genes. One of these differentially expressed genes in rice is a specific amidohydrolase. We characterized this amidohydrolase gene on the rice chromosome 12 as the first plant guanine deaminase (OsGDA1). The biochemical activity of GDA is known from tea and coffee plants where its catalytic product, xanthine, is the precursor for theine and caffeine. However, no plant gene that is coding for GDA is known so far. Recombinant OsGDA1 converted guanine to xanthine in vitro. Measurement of guanine and xanthine contents in the OsGDA1 knockout (KO) line and in the wild type Tainung 67 rice plants also suggested GDA activity in vivo. The content of cellular xanthine is important because of its catabolic products allantoin, ureides, and urea which play roles in water and nitrogen stress tolerance among others. The identification of OsGDA1 fills a critical gap in the S-adenosyl-methionine (SAM) to xanthine pathway. SAM is converted to S-adenosyl-homocysteine (SAH) and finally to xanthine. SAH is a potent inhibitor of DNA methyltransferases, the reduction of which leads to increased DNA methylation and gene silencing in Arabidopsis. We report that the OsGDA1 KO line exhibited a decrease in SAM, SAH and adenosine and an increase in rice genome methylation. The OsGDA1 protein phylogeny combined with mutational protein destabilization analysis suggested artificial selection for null mutants, which could affect genome methylation as in the KO line. Limited information on genes that may affect epigenetics indirectly requires deeper insights into such a role and effect of purine catabolism and related genetic networks.

Original languageEnglish
Pages (from-to)1853-1866
Number of pages14
JournalPhysiologia Plantarum
Volume172
Issue number4
DOIs
StatePublished - Aug 2021

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