A dihydropyridine-sensitive calcium channel in rodent osteoblastic cells

E. Karpinski, L. Wu, R. Civitelli, L. V. Avioli, K. A. Hruska, Peter K.T. Pang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Rat osteogenic sarcoma cells (UMR 106-01) and normal rat trabecular bone osteoblasts (ROB) were studied using the whole cell version of the patch clamp technique to determine the existence of calcium (Ca2+) channels. Pipette and bath solutions were designed to separate Ca2+ channel currents from other voltage-dependent currents, and Ba2+ was used as the charge carrier. In both UMR 106-01 and ROB cells, a Ba2+ current was measured, which expressed the characteristics of an L-channel, such as activation range, dihydropyridine sensitivity, and little or no inactivation. In some cases, this channel was detectable only with BAY-K-8644 in the bath solution. The dihydropyridine agonist increased the current intensity and shifted the peak inward current to more negative potentials. This study, confirming previous observations, demonstrates the existence of a Ca2+ channel in both transformed and normal osteoblastic cells.

Original languageEnglish
Pages (from-to)54-57
Number of pages4
JournalCalcified Tissue International
Volume45
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • Calcium channels
  • Cytosolic calcium concentration
  • Osteoblastic cells

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