TY - JOUR
T1 - A developmental stage-specific promoter directs germline transcription of Dβj̄ gene segments in precursor T lymphocytes
AU - Sikes, Michael L.
AU - Gomez, Key J.
AU - Song, Jing
AU - Oltz, Eugene M.
PY - 1998/8/1
Y1 - 1998/8/1
N2 - The tissue- and stage-specific assembly of Ag receptor genes is regulated by transcriptional control elements positioned within Ig and TCR loci. To further understand the role of cis-acting elements in these regulatory mechanisms, we have characterized a transcriptional promoter that drives germline expression of TCRβ gene segments in vivo. The activity of this promoter, termed PDβ, is restricted to a highly conserved 400-bp region located directly upstream from Dβ1-coding sequences. Maximal PDβ activity requires a TATA element situated within the Dβ1 recombination signal sequences and consensus binding sites for the ubiquitous SP1 and the T cell- specific GATA-3 transcription factors. When linked to active enhancer elements, PDβ directs transcription in most cell types; however, the TCRβ enhancer (Eβ) stimulates PDβ function specifically in precursor T lymphocytes. These findings suggest that PDβ/Eβ interactions may contribute to differential regulation of regions within the TCRβ locus during thymocyte development.
AB - The tissue- and stage-specific assembly of Ag receptor genes is regulated by transcriptional control elements positioned within Ig and TCR loci. To further understand the role of cis-acting elements in these regulatory mechanisms, we have characterized a transcriptional promoter that drives germline expression of TCRβ gene segments in vivo. The activity of this promoter, termed PDβ, is restricted to a highly conserved 400-bp region located directly upstream from Dβ1-coding sequences. Maximal PDβ activity requires a TATA element situated within the Dβ1 recombination signal sequences and consensus binding sites for the ubiquitous SP1 and the T cell- specific GATA-3 transcription factors. When linked to active enhancer elements, PDβ directs transcription in most cell types; however, the TCRβ enhancer (Eβ) stimulates PDβ function specifically in precursor T lymphocytes. These findings suggest that PDβ/Eβ interactions may contribute to differential regulation of regions within the TCRβ locus during thymocyte development.
UR - http://www.scopus.com/inward/record.url?scp=0032145405&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.161.3.1399
DO - 10.4049/jimmunol.161.3.1399
M3 - Article
C2 - 9686603
AN - SCOPUS:0032145405
SN - 0022-1767
VL - 161
SP - 1399
EP - 1405
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -