A detailed phenotypic analysis of immune cell populations in the bronchoalveolar lavage fluid of atopic asthmatics after segmental allergen challenge

Jonathan S. Boomer, Amit D. Parulekar, Brenda M. Patterson, Huiqing Yin-Declue, Christine M. Deppong, Seth Crockford, Nizar N. Jarjour, Mario Castro, Jonathan M. Green

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Abstract

Background: Atopic asthma is characterized by intermittent exacerbations triggered by exposure to allergen. Exacerbations are characterized by an acute inflammatory reaction in the airways, with recruitment of both innate and adaptive immune cells. These cell populations as well as soluble factors are critical for initiating and controlling the inflammatory processes in allergic asthma. Detailed data on the numbers and types of cells recruited following allergen challenge is lacking. In this paper we present an extensive phenotypic analysis of the inflammatory cell infiltrate present in the bronchoalveolar lavage (BAL) fluid following bronchoscopically directed allergen challenge in mild atopic asthmatics.Methods: A re-analysis of pooled data obtained prior to intervention in our randomized, placebo controlled, double blinded study (costimulation inhibition in asthma trial [CIA]) was performed. Twenty-four subjects underwent bronchoscopically directed segmental allergen challenge followed by BAL collection 48 hours later. The BAL fluid was analyzed by multi-color flow cytometry for immune cell populations and multi-plex ELISA for cytokine detection.Results: Allergen instillation induced pro-inflammatory cytokines (IL-6) and immune modulating cytokines (IL-2, IFN-γ, and IL-10) along with an increase in lymphocytes and suppressor cells (Tregs and MDSC). Interestingly, membrane expression of CD30 was identified on lymphocytes, especially Tregs, but not eosinophils. Soluble CD30 was also detected in the BAL fluid after allergen challenge in adult atopic asthmatics.Conclusions: After segmental allergen challenge of adult atopic asthmatics, cell types associated with a pro-inflammatory as well as an anti-inflammatory response are detected within the BAL fluid of the lung.

Original languageEnglish
Article number37
JournalAllergy, Asthma and Clinical Immunology
Volume9
Issue number1
DOIs
StatePublished - Sep 17 2013

Keywords

  • Asthma
  • CD30 expression
  • Segmental allergen challenge
  • T lymphocyte

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    Boomer, J. S., Parulekar, A. D., Patterson, B. M., Yin-Declue, H., Deppong, C. M., Crockford, S., Jarjour, N. N., Castro, M., & Green, J. M. (2013). A detailed phenotypic analysis of immune cell populations in the bronchoalveolar lavage fluid of atopic asthmatics after segmental allergen challenge. Allergy, Asthma and Clinical Immunology, 9(1), [37]. https://doi.org/10.1186/1710-1492-9-37