TY - JOUR
T1 - A defective interfering RNA that contains a mosaic of a plant virus genome
AU - Hillman, Bradley I.
AU - Carrington, James C.
AU - Morris, Thomas J.
N1 - Funding Information:
The authors with to thank Dr. A. 0. Jackson and Dr. G. Bruening for helpful discussions, and Dr. Louis Heaton for help with computer analysis. Mr. Patrick Hearne is acknowledged for his sequencing of a portion of the 5’-proximal region of the TBSV genome. We thank Mr. Bruce Malcolm for synthesis of oligonucleotides. This research was sup ported in part by a USDA Competitive Grant (#83-CRCR-1-1227).
PY - 1987/11/6
Y1 - 1987/11/6
N2 - A symptom-modulating RNA associated with tomato bushy stunt virus (TBSV) was investigated with respect to physical and biological properties. Linear RNA of approximately 396 nucleotides was packaged in viral coat protein and was dependent on TBSV for replication. Coinoculation of the small RNA with TBSV resulted in the attenuation of TBSV-induced symptoms and depression of virus synthesis in whole plants. Nucleotide sequence analysis revealed that the symptom-modulating RNA was derived from 5′, 3′, and internal segments of the TBSV genome. The identification of this symptom-modulating RNA as a colinear deletion mutant of the helper virus genome establishes it as the first definitive defective interfering RNA (DI RNA) to be identified in association with a plant virus.
AB - A symptom-modulating RNA associated with tomato bushy stunt virus (TBSV) was investigated with respect to physical and biological properties. Linear RNA of approximately 396 nucleotides was packaged in viral coat protein and was dependent on TBSV for replication. Coinoculation of the small RNA with TBSV resulted in the attenuation of TBSV-induced symptoms and depression of virus synthesis in whole plants. Nucleotide sequence analysis revealed that the symptom-modulating RNA was derived from 5′, 3′, and internal segments of the TBSV genome. The identification of this symptom-modulating RNA as a colinear deletion mutant of the helper virus genome establishes it as the first definitive defective interfering RNA (DI RNA) to be identified in association with a plant virus.
UR - http://www.scopus.com/inward/record.url?scp=0023646739&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(87)90638-6
DO - 10.1016/0092-8674(87)90638-6
M3 - Article
C2 - 3664641
AN - SCOPUS:0023646739
SN - 0092-8674
VL - 51
SP - 427
EP - 433
JO - Cell
JF - Cell
IS - 3
ER -