A CTP-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels

Moritz Ammelburg, Marcus D. Hartmann, Sergej Djuranovic, Vikram Alva, Kristin K. Koretke, Jörg Martin, Guido Sauer, Vincent Truffault, Kornelius Zeth, Andrei N. Lupas, Murray Coles

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17 Scopus citations

Abstract

Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved βαβ-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases.

Original languageEnglish
Pages (from-to)1577-1590
Number of pages14
JournalStructure
Volume15
Issue number12
DOIs
StatePublished - Dec 13 2007
Externally publishedYes

Keywords

  • PROTEINS

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    Ammelburg, M., Hartmann, M. D., Djuranovic, S., Alva, V., Koretke, K. K., Martin, J., Sauer, G., Truffault, V., Zeth, K., Lupas, A. N., & Coles, M. (2007). A CTP-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels. Structure, 15(12), 1577-1590. https://doi.org/10.1016/j.str.2007.09.027