A critical role for the peroxisome proliferator-activated receptor α (PPARα) in the cellular fasting response: The PPARα-null mouse as a model of fatty acid oxidation disorders

Teresa C. Leone, Carla J. Weinheimer, Daniel P. Kelly

Research output: Contribution to journalArticlepeer-review

811 Scopus citations

Abstract

We hypothesized that the lipid-activated transcription factor, the peroxisome proliferator-activated receptor α (PPARα), plays a pivotal role in the cellular metabolic response to fasting. Short-term starvation caused hepatic steatosis, myocardial lipid accumulation, and hypoglycemia, with an inadequate ketogenic response in adult mice lacking PPARα (PPARα(-/-), a phenotype that bears remarkable similarity to that of humans with genetic defects in mitochondrial fatty acid oxidation enzymes. In PPARα(+/+) mice, fasting induced the hepatic and cardiac expression of PPARα target genes encoding key mitochondrial (medium-chain acyl-CoA dehydrogenase, carnitine palmitoyltransferase I) and extramitochondrial (acyl-CoA oxidase, cytochrome P450 4A3) enzymes. In striking contrast, the hepatic and cardiac expression of most PPARα target genes was not induced by fasting in PPARα(-/-) mice. These results define a critical role for PPARα in a transcriptional regulatory response to fasting and identify the PPARα(-/-) mouse as a potentially useful murine model of inborn and acquired abnormalities of human fatty acid utilization.

Original languageEnglish
Pages (from-to)7473-7478
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number13
DOIs
StatePublished - Jun 22 1999

Keywords

  • Dietary regulation
  • Lipid metabolism
  • Nuclear hormone receptors
  • Transcriptional control

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