TY - JOUR
T1 - A counterdefensive strategy of plant viruses
T2 - Suppression of posttranscriptional gene silencing
AU - Kasschau, Kristin D.
AU - Carrington, James C.
N1 - Funding Information:
We thank Bill Dougherty for providing invaluable expertise and insight into this work and for collaborating on production of the initial GUS transgenic lines. We thank Jeanmarie Verchot for production of the GUS mutant used to prepare transgenic plants, Quyen Vo for helping with susceptibility screens, and Steven Whitham for constructive comments on the manuscript. Thom Koehler and Mari Greaney are thanked for providing excellent greenhouse support. This work was supported by grants from the U. S. Department of Agriculture (NRI 95–37303–1867) and the National Institutes of Health (AI27832 and AI43288).
PY - 1998/11/13
Y1 - 1998/11/13
N2 - Posttranscriptional gene silencing (PTGS) in plants inactivates some aberrant or highly expressed RNAs in a sequence-specific manner in the cytoplasm. A silencing mechanism similar to PTGS appears to function as an adaptive antiviral response. We demonstrate that the P1/HC-Pro polyprotein encoded by tobacco etch virus functions as a suppressor of PTGS. A locus comprised of a highly expressed β-glucuronidase (GUS) transgene was shown to exhibit PTGS. Genetic crosses and segregation analyses revealed that a P1/HC- Pro transgene suppressed PTGS of the GUS sequence. Nuclear transcription assays indicated that the silencing suppression activity of P1/HC-Pro was at the posttranscriptional level. These data reveal that plant viruses can condition enhanced susceptibility within a host through interdiction of a potent defense response.
AB - Posttranscriptional gene silencing (PTGS) in plants inactivates some aberrant or highly expressed RNAs in a sequence-specific manner in the cytoplasm. A silencing mechanism similar to PTGS appears to function as an adaptive antiviral response. We demonstrate that the P1/HC-Pro polyprotein encoded by tobacco etch virus functions as a suppressor of PTGS. A locus comprised of a highly expressed β-glucuronidase (GUS) transgene was shown to exhibit PTGS. Genetic crosses and segregation analyses revealed that a P1/HC- Pro transgene suppressed PTGS of the GUS sequence. Nuclear transcription assays indicated that the silencing suppression activity of P1/HC-Pro was at the posttranscriptional level. These data reveal that plant viruses can condition enhanced susceptibility within a host through interdiction of a potent defense response.
UR - http://www.scopus.com/inward/record.url?scp=0032514856&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81614-1
DO - 10.1016/S0092-8674(00)81614-1
M3 - Article
C2 - 9827799
AN - SCOPUS:0032514856
SN - 0092-8674
VL - 95
SP - 461
EP - 470
JO - Cell
JF - Cell
IS - 4
ER -