A comprehensive promoter landscape identifies a novel promoter for CD133+ in restricted tissues, cancers, and stem cells

Ramakrishna Sompallae, Oliver Hofmann, Christopher A. Maher, Craig Gedye, Andreas Behren, Morana Vitezic, Carsten O. Daub, Sylvie Devalle, Otavia L. Caballero, Piero Carninci, Yoshihide Hayashizaki, Elizabeth R. Lawlor, Jonathan Cebon, Winston Hide

Research output: Contribution to journalReview article

7 Scopus citations

Abstract

PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133+ melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133+ enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.

Original languageEnglish
Article number209
JournalFrontiers in Genetics
Volume4
Issue numberOCT
DOIs
StatePublished - Dec 1 2013

Keywords

  • AC antigen
  • Cancer stem cells
  • Genetic
  • Human
  • Melanoma
  • PROM1 protein
  • Transcription start site
  • promoter regions

Fingerprint Dive into the research topics of 'A comprehensive promoter landscape identifies a novel promoter for CD<sub>133+</sub> in restricted tissues, cancers, and stem cells'. Together they form a unique fingerprint.

Cite this