A comparison of two anesthetic techniques for the study of rat skeletal muscle high-energy phosphates in vivo using 31P-NMR

Steven F. Loy, Walter A. Boyle, Leigh D. Segel

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

31P-Nuclear magnetic resonance (NMR) spectroscopy was used to evaluate in vivo rat quadriceps ATP, phosphocreatine (PCr), inorganic phosphate (Pi) and tissue pH during anesthesia with ketamine/xylazine (K/X) or isoflurane (IS). A surface coil was used to receive signals from the quadriceps muscle of rats positioned in a wide-bore horizontal magnet. The PCr/β-ATP ratios determined from the NMR spectra were 4.34 ± 0.19 (K/X) and 4.40 ± 0.28 (IS). Tissue pH was 7.09 ± 0.05 (K/X) and 7.13 ± 0.07 (IS). Metabolic stability of quadriceps PCr and ATP was demonstrated during both K/X and IS anesthesia, but the K/X-anesthetized animals had longer sleep time, lower food consumption, and lower body weight post-anesthesia than the IS-anesthetized animals. The PCr/β-ATP ratio in quadriceps of repetitively IS-anesthetized rats did not fluctuate diurnally. In addition, the animals recovered rapidly and continued to gain weight following the multiple brief IS anesthetic procedures. These data indicate that serial in vivo investigations of high-energy-phosphate metabolism in small animals can be accomplished using 31P-NMR spectroscopy and IS anesthesia, which has several advantages over K/X anesthesia for these types of studies.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalBBA - General Subjects
Volume964
Issue number3
DOIs
StatePublished - Mar 17 1988

Keywords

  • (Rat skeletal muscle)
  • Anesthetic
  • High energy phosphate
  • Isoflurane
  • Ketamine
  • NMR, P-
  • Xylazine

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