TY - JOUR
T1 - A cloned Drosophila DNA fragment which codes for a 4 S RNA species
AU - Schedl, Tim B.
AU - Donelson, John E.
N1 - Funding Information:
Some of this work formed part of an undergraduate research thesis project by T.B.S. at Lawrence University, Appleton, Wisc. We are both indebted to S. Artavanis-Tsakonas, E. Gubbins, W. Perreault, P. Schedl and R. Stewart for technical and experimental advice as well as to those who generously provide us with strains and materials. Most of the financial support was from N.I.H. grant GM-21696 and N.S.F. grant PCM 76-13461.
PY - 1978/10/24
Y1 - 1978/10/24
N2 - A collection of random Drosophila melanogaster DNA fragments cloned individually in Escherichia coli was screened for the presence of sequences complementary to the 4 S, 5 S and 5.8 S RNA species produced in the D. melanogaster Kc tissue culture line. Four D. melanogaster DNA fragments were found which possessed sequences complementary to the 4 S RNA species but not complementary to the 5 S or 5.8 S RNA. One such cloned fragment (6.81 kilobase in length) was characterized further. It hybridizes in situ to region 22A-C of the left arm of chromosome 2 and does not contain repetitive sequences detectable by renaturation (c0t) analysis. This same region was reported earlier by Steffensen and Wimber (Genetics (1971) 69, 163-178) to hybridize in situ to bulk tRNA extracted from D. melanogaster.
AB - A collection of random Drosophila melanogaster DNA fragments cloned individually in Escherichia coli was screened for the presence of sequences complementary to the 4 S, 5 S and 5.8 S RNA species produced in the D. melanogaster Kc tissue culture line. Four D. melanogaster DNA fragments were found which possessed sequences complementary to the 4 S RNA species but not complementary to the 5 S or 5.8 S RNA. One such cloned fragment (6.81 kilobase in length) was characterized further. It hybridizes in situ to region 22A-C of the left arm of chromosome 2 and does not contain repetitive sequences detectable by renaturation (c0t) analysis. This same region was reported earlier by Steffensen and Wimber (Genetics (1971) 69, 163-178) to hybridize in situ to bulk tRNA extracted from D. melanogaster.
UR - http://www.scopus.com/inward/record.url?scp=0018143119&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(78)90140-5
DO - 10.1016/0005-2787(78)90140-5
M3 - Article
C2 - 102349
AN - SCOPUS:0018143119
SN - 0005-2787
VL - 520
SP - 539
EP - 554
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 3
ER -