A collection of random Drosophila melanogaster DNA fragments cloned individually in Escherichia coli was screened for the presence of sequences complementary to the 4 S, 5 S and 5.8 S RNA species produced in the D. melanogaster Kc tissue culture line. Four D. melanogaster DNA fragments were found which possessed sequences complementary to the 4 S RNA species but not complementary to the 5 S or 5.8 S RNA. One such cloned fragment (6.81 kilobase in length) was characterized further. It hybridizes in situ to region 22A-C of the left arm of chromosome 2 and does not contain repetitive sequences detectable by renaturation (c0t) analysis. This same region was reported earlier by Steffensen and Wimber (Genetics (1971) 69, 163-178) to hybridize in situ to bulk tRNA extracted from D. melanogaster.

Original languageEnglish
Pages (from-to)539-554
Number of pages16
JournalBBA Section Nucleic Acids And Protein Synthesis
Issue number3
StatePublished - Oct 24 1978


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