TY - JOUR
T1 - A Casz1–NuRD complex regulates temporal identity transitions in neural progenitors
AU - Mattar, Pierre
AU - Jolicoeur, Christine
AU - Dang, Thanh
AU - Shah, Sujay
AU - Clark, Brian S.
AU - Cayouette, Michel
N1 - Funding Information:
We thank Seth Blackshaw, and Johan Ericsson for sharing antibodies. We thank Jean-François Côté for help with BioID and 293T-REx cells. We thank Jessica Barthe and Marie-Claude Lavallée for help with animal husbandry. We also thank Denis Faubert and Josée Champagne of the IRCM proteomic core facility for mass spectrometry, and members of the Cayouette and Mattar labs for insightful discussions. We thank Alejandro Chavez, Connie Cepko, George Church, Ann-Claude Gingras, and Feng Zhang for sharing plasmids. This work was supported by the Canadian Institutes of Health Research (CIHR, FDN 159936; PJT 166032) and Fighting Blindness Canada. P.M. was supported by a CIHR Postdoctoral Fellowship and currently holds the Gladys and Lorna J. Wood Chair for Research in Vision. M.C. holds the Gaëtane and Roland Pillenière Chair in Retina Biology and is a Research Scholar Emeritus of the Fonds de recherche du Québec—Santé. B.S.C. is supported by grants from the NIH (R00EY027844) and an unrestricted grant to the Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Neural progenitor cells undergo identity transitions during development to ensure the generation different types of neurons and glia in the correct sequence and proportions. A number of temporal identity factors that control these transitions in progenitor competence have been identified, but the molecular mechanisms underlying their function remain unclear. Here, we asked how Casz1, the mammalian orthologue of Drosophila castor, regulates competence during retinal development. We show that Casz1 is required to control the transition between neurogenesis and gliogenesis. Using BioID proteomics, we reveal that Casz1 interacts with the nucleosome remodeling and deacetylase (NuRD) complex in retinal cells. Finally, we show that both the NuRD and the polycomb repressor complexes are required for Casz1 to promote the rod fate and suppress gliogenesis. As additional temporal identity factors have been found to interact with the NuRD complex in other contexts, we propose that these factors might act through this common biochemical process to regulate neurogenesis.
AB - Neural progenitor cells undergo identity transitions during development to ensure the generation different types of neurons and glia in the correct sequence and proportions. A number of temporal identity factors that control these transitions in progenitor competence have been identified, but the molecular mechanisms underlying their function remain unclear. Here, we asked how Casz1, the mammalian orthologue of Drosophila castor, regulates competence during retinal development. We show that Casz1 is required to control the transition between neurogenesis and gliogenesis. Using BioID proteomics, we reveal that Casz1 interacts with the nucleosome remodeling and deacetylase (NuRD) complex in retinal cells. Finally, we show that both the NuRD and the polycomb repressor complexes are required for Casz1 to promote the rod fate and suppress gliogenesis. As additional temporal identity factors have been found to interact with the NuRD complex in other contexts, we propose that these factors might act through this common biochemical process to regulate neurogenesis.
UR - http://www.scopus.com/inward/record.url?scp=85100873333&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-83395-7
DO - 10.1038/s41598-021-83395-7
M3 - Article
C2 - 33594190
AN - SCOPUS:85100873333
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 3858
ER -