Barajas-Mora et al. demonstrate that an enhancer—E88—in the V gene region of the Igκ locus regulates long-range chromatin interactions, shaping its 3D structure, enabling different V-gene-containing subTADs to come in proximity to the J genes to generate a diverse repertoire. Consequently, E88 deletion perturbs the resulting B cell repertoire.
- 3D structure
- B cells
- V(D)J recombination
- antibody repertoire
- chromatin conformation capture
- long-range chromatin interactions
- nuclear topology