A 3D, Magnetically Actuated, Aligned Collagen Fiber Hydrogel Platform Recapitulates Physical Microenvironment of Myoblasts for Enhancing Myogenesis

Nianyuan Shi, Yuhui Li, Le Chang, Guoxu Zhao, Guorui Jin, Yi Lyu, Guy M. Genin, Yufei Ma, Feng Xu

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Many cell responses that underlie the development, maturation, and function of tissues are guided by the architecture and mechanical loading of the extracellular matrix (ECM). Because mechanical stimulation must be transmitted through the ECM architecture, the synergy between these two factors is important. However, recapitulating the synergy of these physical microenvironmental cues in vitro remains challenging. To address this, a 3D magnetically actuated collagen hydrogel platform is developed that enables combined control of ECM architecture and mechanical stimulation. With this platform, it is demonstrated how these factors synergistically promote cell alignment of C2C12 myoblasts and enhance myogenesis. This promotion is driven in part by the dynamics of Yes-associated protein and structure of cellular microtubule networks. This facile platform holds great promises for regulating cell behavior and fate, generating a broad range of engineered physiologically representative microtissues in vitro, and quantifying the mechanobiology underlying their functions.

Original languageEnglish
Article number2100276
JournalSmall Methods
Volume5
Issue number6
DOIs
StatePublished - Jun 15 2021

Keywords

  • 3D aligned hydrogels
  • cell mechanical microenvironment
  • functional microtissues
  • magnetic hydrogels
  • myogenesis

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