@article{8d68ddcedcd54a3484a4ce4d0d02cbae,
title = "46 kd mannose 6-phosphate receptor: Cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor",
abstract = "We have isolated cDNA clones encoding the entire sequence of the bovine 46 kd cation-dependent mannose 6-phosphate (CD Man-6-P) receptor. Translation of CD Man-6-P receptor mRNA in Xenopus laevis oocytes results in a protein that binds specifically to phosphomannan-Sepharose, thus demonstrating that our cDNA clones encode a functional receptor. The deduced 279 amino acid sequence reveals a single polypeptide chain that contains a putative signal sequence and a transmembrane domain. Trypsin digestion of microsomal membranes containing the receptor and the location of the five potential N-linked glycosylation sites indicate that the receptor is a transmembrane protein with an extracytoplasmic amino terminus. This extracytoplasmic domain is homologous to the approximately 145 amino acid long repeating domains present in the 215 kd cation-independent Man-6-P receptor.",
author = "Dahms, {Nancy M.} and Peter Lobel and James Breitmeyer and Chirgwin, {John M.} and Stuart Kornfeld",
note = "Funding Information: We thank Gregory A. Grant and Mark Frazier of the Washington Universrty Protein Facility for performing the peptide analysis, and Peter S. Rotwein for generously providing the bovine calf liver cDNA II-brary. We are also very grateful to Walter Gregory for the CD Man-6-P receptor-specific antisera, to Bernard Hoflack for providing purified CD Man-6-P receptor, and to Phyllis Faust for helpful advice concerning the oocyte injections and for generously providing the cathepsrn D poly(A) tail. Thisinvestigation was supported by grants ROl CA08759 and HL33933 from the United States Public Health Service, grant 83. 1271 from the American Heart Association, and a Monsanto Company/Washington University Biomedical Research Grant. N. M. D. and P. L. are supported by American Cancer Society fellowships PF-2873 and PF-2777.",
year = "1987",
month = jul,
day = "17",
doi = "10.1016/0092-8674(87)90214-5",
language = "English",
volume = "50",
pages = "181--192",
journal = "Cell",
issn = "0092-8674",
number = "2",
}