3′,5′-Cyclic dinucleoside phosphates: Undesirable intermediates in the phosphotriester approach to oligonucleotide synthesis

Jacques H. Van Boom, Peter M.J. Burgers, Peter H. Van Deursen, Jan F.M. De Rooy, Colin B. Reese

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Mild alkaline hydrolysis of (4a) gives the 3′,5′-cyclic dinucleoside phosphate (5a); more drastic alkaline hydrolysis of phosphotriester intermediates with free vicinal 5′- or 3′-hydroxy functions [(4a) and (7b), or (6a) and (8b)] leads to products with, respectively, 5′ → 5′- or 3′ → 3′-in addition to 3′ → 5′-internucleotide linkages.

Original languageEnglish
Pages (from-to)167-168
Number of pages2
JournalJournal of the Chemical Society, Chemical Communications
Issue number5
DOIs
StatePublished - Dec 1 1976

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