3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes

Shupei Wang; Joseph H. Nadeau; Alessandra Duncan; Marie France Robert; Gisèle Fontaine; Keith Schappert; Kenneth R. Johnson; Ewa Zietkiewicz; Paul Hruz; Henry Miziorko; Grant A. Mitchell

doi:10.1007/BF00360589

3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes

Shupei Wang, Joseph H. Nadeau, Alessandra Duncan, Marie France Robert, Gisèle Fontaine, Keith Schappert, Kenneth R. Johnson, Ewa Zietkiewicz, Paul Hruz, Henry Miziorko, Grant A. Mitchell

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Abstract

3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL) is a homodimeric mitochondrial matrix enzyme that catalyzes the last step of ketogenesis. Using a human HL cDNA as a probe, we isolated a 1.4-kb mouse HL cDNA (HLM) from a mouse liver library and extended the sequence in the 5′ direction, using RACE PCR to include the complete coding sequence. The nucleotide sequence of the mouse HL coding region is 85.7% identical to human HL, and 52.6% to Ps. mevalonii HL. Peptide identities of 87.4% and 54.3% respectively were observed. Southern analysis of 29 strains of laboratory mice and of Mus spretus revealed a total of about 25 kb of hybridizing fragments and three polymorphic fragments in both EcoRI and HindIII digestions. The mouse HL locus (Hmgcl) was localized on Chromosome (Chr) 4: Pmv-19-12.6±3.6 cM-Hmgcl-7.3±2.3 cM-Xmv-8-1.5±1.0 cM-Gpd1. The human HL locus (HMGCL) was mapped to distal Chr 1p by analysis of a human-hamster hybrid cell panel and by in situ hybridization.

Original language	English
Pages (from-to)	382-387
Number of pages	6
Journal	Mammalian Genome
Volume	4
Issue number	7
DOIs	https://doi.org/10.1007/BF00360589
State	Published - Jul 1993

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Wang, S., Nadeau, J. H., Duncan, A., Robert, M. F., Fontaine, G., Schappert, K., Johnson, K. R., Zietkiewicz, E., Hruz, P., Miziorko, H., & Mitchell, G. A. (1993). 3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes. Mammalian Genome, 4(7), 382-387. https://doi.org/10.1007/BF00360589

@article{3178bd8312ad4a0ebcdc8e1e29f3cc3f,

title = "3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes",

abstract = "3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL) is a homodimeric mitochondrial matrix enzyme that catalyzes the last step of ketogenesis. Using a human HL cDNA as a probe, we isolated a 1.4-kb mouse HL cDNA (HLM) from a mouse liver library and extended the sequence in the 5′ direction, using RACE PCR to include the complete coding sequence. The nucleotide sequence of the mouse HL coding region is 85.7% identical to human HL, and 52.6% to Ps. mevalonii HL. Peptide identities of 87.4% and 54.3% respectively were observed. Southern analysis of 29 strains of laboratory mice and of Mus spretus revealed a total of about 25 kb of hybridizing fragments and three polymorphic fragments in both EcoRI and HindIII digestions. The mouse HL locus (Hmgcl) was localized on Chromosome (Chr) 4: Pmv-19-12.6±3.6 cM-Hmgcl-7.3±2.3 cM-Xmv-8-1.5±1.0 cM-Gpd1. The human HL locus (HMGCL) was mapped to distal Chr 1p by analysis of a human-hamster hybrid cell panel and by in situ hybridization.",

author = "Shupei Wang and Nadeau, {Joseph H.} and Alessandra Duncan and Robert, {Marie France} and Gis{\`e}le Fontaine and Keith Schappert and Johnson, {Kenneth R.} and Ewa Zietkiewicz and Paul Hruz and Henry Miziorko and Mitchell, {Grant A.}",

year = "1993",

month = jul,

doi = "10.1007/BF00360589",

language = "English",

volume = "4",

pages = "382--387",

journal = "Mammalian Genome",

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number = "7",

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Wang, S, Nadeau, JH, Duncan, A, Robert, MF, Fontaine, G, Schappert, K, Johnson, KR, Zietkiewicz, E, Hruz, P, Miziorko, H & Mitchell, GA 1993, '3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes', Mammalian Genome, vol. 4, no. 7, pp. 382-387. https://doi.org/10.1007/BF00360589

TY - JOUR

T1 - 3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL)

T2 - cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes

AU - Wang, Shupei

AU - Nadeau, Joseph H.

AU - Duncan, Alessandra

AU - Robert, Marie France

AU - Fontaine, Gisèle

AU - Schappert, Keith

AU - Johnson, Kenneth R.

AU - Zietkiewicz, Ewa

AU - Hruz, Paul

AU - Miziorko, Henry

AU - Mitchell, Grant A.

PY - 1993/7

Y1 - 1993/7

N2 - 3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL) is a homodimeric mitochondrial matrix enzyme that catalyzes the last step of ketogenesis. Using a human HL cDNA as a probe, we isolated a 1.4-kb mouse HL cDNA (HLM) from a mouse liver library and extended the sequence in the 5′ direction, using RACE PCR to include the complete coding sequence. The nucleotide sequence of the mouse HL coding region is 85.7% identical to human HL, and 52.6% to Ps. mevalonii HL. Peptide identities of 87.4% and 54.3% respectively were observed. Southern analysis of 29 strains of laboratory mice and of Mus spretus revealed a total of about 25 kb of hybridizing fragments and three polymorphic fragments in both EcoRI and HindIII digestions. The mouse HL locus (Hmgcl) was localized on Chromosome (Chr) 4: Pmv-19-12.6±3.6 cM-Hmgcl-7.3±2.3 cM-Xmv-8-1.5±1.0 cM-Gpd1. The human HL locus (HMGCL) was mapped to distal Chr 1p by analysis of a human-hamster hybrid cell panel and by in situ hybridization.

AB - 3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL) is a homodimeric mitochondrial matrix enzyme that catalyzes the last step of ketogenesis. Using a human HL cDNA as a probe, we isolated a 1.4-kb mouse HL cDNA (HLM) from a mouse liver library and extended the sequence in the 5′ direction, using RACE PCR to include the complete coding sequence. The nucleotide sequence of the mouse HL coding region is 85.7% identical to human HL, and 52.6% to Ps. mevalonii HL. Peptide identities of 87.4% and 54.3% respectively were observed. Southern analysis of 29 strains of laboratory mice and of Mus spretus revealed a total of about 25 kb of hybridizing fragments and three polymorphic fragments in both EcoRI and HindIII digestions. The mouse HL locus (Hmgcl) was localized on Chromosome (Chr) 4: Pmv-19-12.6±3.6 cM-Hmgcl-7.3±2.3 cM-Xmv-8-1.5±1.0 cM-Gpd1. The human HL locus (HMGCL) was mapped to distal Chr 1p by analysis of a human-hamster hybrid cell panel and by in situ hybridization.

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U2 - 10.1007/BF00360589

DO - 10.1007/BF00360589

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AN - SCOPUS:0027520577

SN - 0938-8990

VL - 4

SP - 382

EP - 387

JO - Mammalian Genome

JF - Mammalian Genome

IS - 7

ER -

3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and characterization of a mouse liver HL cDNA and subchromosomal mapping of the human and mouse HL genes

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