2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803

Birgitta Norling; Elena Zak; Bertil Andersson; Himadri Pakrasi

doi:10.1016/S0014-5793(98)01123-5

2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803

Birgitta Norling
, Elena Zak
, Bertil Andersson
, Himadri Pakrasi

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	189-192
Number of pages	4
Journal	FEBS Letters
Volume	436
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(98)01123-5
State	Published - Oct 2 1998

Keywords

Cyanobacterium
Membrane protein
Phase partitioning
Proteomics
Synechocystis 6803

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10.1016/S0014-5793(98)01123-5

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@article{e92f4daa3bbe4842b726b3ff993aa755,

title = "2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803",

abstract = "Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Cyanobacterium, Membrane protein, Phase partitioning, Proteomics, Synechocystis 6803",

author = "Birgitta Norling and Elena Zak and Bertil Andersson and Himadri Pakrasi",

note = "Funding Information: We thank Dr. T. Omata for the gift of antibodies against the NrtA protein. Support for this work has been provided by the International Human Frontier Sciences Program (B.A. and H.P.), The Carl Trygger Foundation (B.N.), and the US National Science Foundation (H.P.).",

year = "1998",

month = oct,

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doi = "10.1016/S0014-5793(98)01123-5",

language = "English",

volume = "436",

pages = "189--192",

journal = "FEBS Letters",

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T1 - 2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803

AU - Norling, Birgitta

AU - Zak, Elena

AU - Andersson, Bertil

AU - Pakrasi, Himadri

N1 - Funding Information: We thank Dr. T. Omata for the gift of antibodies against the NrtA protein. Support for this work has been provided by the International Human Frontier Sciences Program (B.A. and H.P.), The Carl Trygger Foundation (B.N.), and the US National Science Foundation (H.P.).

PY - 1998/10/2

Y1 - 1998/10/2

N2 - Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - Cyanobacterium

KW - Membrane protein

KW - Phase partitioning

KW - Proteomics

KW - Synechocystis 6803

UR - https://www.scopus.com/pages/publications/0032475858

U2 - 10.1016/S0014-5793(98)01123-5

DO - 10.1016/S0014-5793(98)01123-5

M3 - Article

C2 - 9781676

AN - SCOPUS:0032475858

SN - 0014-5793

VL - 436

SP - 189

EP - 192

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803

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Keywords

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