TY - JOUR
T1 - 25-Hydroxycholecalciferol
T2 - Specific binding by rachitic tissue extracts
AU - Haddad, John G.
AU - Birge, Stanley J.
N1 - Funding Information:
This work was supported in part by grants AM
PY - 1971/11/19
Y1 - 1971/11/19
N2 - In vitro incubation of 3H-25-hydroxycholecalciferol with 105,000 x g supernatants from homogenates of rachitic rat kidney and skeletal muscle resulted in sedimentation of radioactivity at 5-6 S, in contrast to the 3-4 S sedimentation observed when the sterol was incubated with rachitic serum. The binding of 3H-25-hydroxycholecalciferol by these tissue extracts was reduced by exposure to anti-rachitic sterols containing an open B ring with C10 methylene and C9 hydrogenation, and by pre-incubation of the extracts with trypsin.
AB - In vitro incubation of 3H-25-hydroxycholecalciferol with 105,000 x g supernatants from homogenates of rachitic rat kidney and skeletal muscle resulted in sedimentation of radioactivity at 5-6 S, in contrast to the 3-4 S sedimentation observed when the sterol was incubated with rachitic serum. The binding of 3H-25-hydroxycholecalciferol by these tissue extracts was reduced by exposure to anti-rachitic sterols containing an open B ring with C10 methylene and C9 hydrogenation, and by pre-incubation of the extracts with trypsin.
UR - http://www.scopus.com/inward/record.url?scp=0015146514&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(71)90413-X
DO - 10.1016/0006-291X(71)90413-X
M3 - Article
C2 - 4330141
AN - SCOPUS:0015146514
SN - 0006-291X
VL - 45
SP - 829
EP - 834
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -