TY - JOUR
T1 - 22-oxacalcitriol suppresses 25-hydroxycholecalciferol-1α-hydroxylase in rat kidney
AU - Grieff, M.
AU - Dusso, A.
AU - Mori, T.
AU - Nishii, Y.
AU - Slatopolsky, E.
AU - Brown, A. J.
N1 - Funding Information:
ACKNOWLEDGMENTS Dr. Grieff is a recipient of a fellowship from the Kidney Foundation of Canada. This work was supported in part by U.S. Public Health Service NIADDK Grants DK-09976 and DK-07126 and a grant provided by Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
PY - 1992/5/29
Y1 - 1992/5/29
N2 - 22-oxacalcitriol can decrease the serum level of 1,25-dihydroxycholecalciferol by increasing its metabolic clearance rate and decreasing its production rate. To determine whether 22-oxacalcitriol suppressed the renal 25-hydroxycholecalciferol-1α-hydroxylase we treated rats with 200 ng of 22-oxacalcitriol daily for 1 week. Enzyme activity was measured in vitro by measurement of production of 1,25-dihydroxycholecalciferol by renal slices incubated with 25-hydroxycholecalciferol. 22-oxacalcitriol significantly decreased the 25-hydroxycholecalciferol-1α-hydroxylase activity from 797 ± 208 pg of 1,25-dihydroxycholecalciferol/g of kidney/h in the control group to 257 ± 150 pg of 1,25-dihydroxycholecalciferol/g of kidney/h (p < 0.05). This is the first demonstration of suppression of the renal 25-hydroxycholecalciferol-1α-hydroxylase by the vitamin D3 analog, 22-oxacalcitriol.
AB - 22-oxacalcitriol can decrease the serum level of 1,25-dihydroxycholecalciferol by increasing its metabolic clearance rate and decreasing its production rate. To determine whether 22-oxacalcitriol suppressed the renal 25-hydroxycholecalciferol-1α-hydroxylase we treated rats with 200 ng of 22-oxacalcitriol daily for 1 week. Enzyme activity was measured in vitro by measurement of production of 1,25-dihydroxycholecalciferol by renal slices incubated with 25-hydroxycholecalciferol. 22-oxacalcitriol significantly decreased the 25-hydroxycholecalciferol-1α-hydroxylase activity from 797 ± 208 pg of 1,25-dihydroxycholecalciferol/g of kidney/h in the control group to 257 ± 150 pg of 1,25-dihydroxycholecalciferol/g of kidney/h (p < 0.05). This is the first demonstration of suppression of the renal 25-hydroxycholecalciferol-1α-hydroxylase by the vitamin D3 analog, 22-oxacalcitriol.
UR - http://www.scopus.com/inward/record.url?scp=0026772697&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)80974-X
DO - 10.1016/S0006-291X(05)80974-X
M3 - Article
C2 - 1599456
AN - SCOPUS:0026772697
SN - 0006-291X
VL - 185
SP - 191
EP - 196
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -