[19-14C]androstenedione: A new substrate for assaying aromatase and studying its reaction mechanism

Douglas F. Covey, Patrick C. McMullan, Linda L. Wixler, Mayo Cabell

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

[19-14C]Androstenedione has been prepared and utilized as a substrate for assaying microsomal human placental aromatase. Enzyme activity is determined by measuring the rate at which [14C]formate is produced by aromatization of this 14C-labeled steroid. Isotope ratio experiments using [19-14C]androstenedione and [1β-3H]androstenedione demonstrate that an apparent kinetic hydrogen isotope effect exists for the aromatization of the tritiated steroid with kH/kT ≈ 1.09. Metabolic switching occurs to a minor extent (≈3%) during aromatization of [1β-3H]androstenedione, but not during the aromatization of [19-14C]androstenedione.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number1
DOIs
StatePublished - Nov 30 1988

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