TY - JOUR
T1 - 1,25-Dihydroxyvitamin D3 induces monocytic differentiation of the PLB-985 leukemic line and promotes c-fgr mRNA expression
AU - Perkins, S. L.
AU - Link, D. C.
AU - Kling, S.
AU - Ley, T. J.
AU - Teitelbaum, S. L.
PY - 1991
Y1 - 1991
N2 - 1,25-dihydroxyvitamin D3 [1,25(OH)2D] is known to stimulate maturation of the human promyelocytic line HL-60 and murine bone marrow precursor cells along a monocyte/macrophage pathway. In this study, the steroid's effects on the PLB-985 leukemic line were examined. We found that 1,25(OH)2D induces monocytic differentiation of PLB-985 as manifested by morphological appearance, histochemical staining, and changes in cell surface antigen expression. Additionally, there was acquisition of functional monocyte characteristics including phagocytic activity and superoxide anion production via the respiratory burst pathway. Steady state levels of mRNA derived from the leukocyte-specific proto-oncogene c-fgr were also increased by the steroid. Thus, 1,25(OH)2D effectively differentiates PLB-985 cells along a monocytic pathway, providing a useful model of macrophage differentiation.
AB - 1,25-dihydroxyvitamin D3 [1,25(OH)2D] is known to stimulate maturation of the human promyelocytic line HL-60 and murine bone marrow precursor cells along a monocyte/macrophage pathway. In this study, the steroid's effects on the PLB-985 leukemic line were examined. We found that 1,25(OH)2D induces monocytic differentiation of PLB-985 as manifested by morphological appearance, histochemical staining, and changes in cell surface antigen expression. Additionally, there was acquisition of functional monocyte characteristics including phagocytic activity and superoxide anion production via the respiratory burst pathway. Steady state levels of mRNA derived from the leukocyte-specific proto-oncogene c-fgr were also increased by the steroid. Thus, 1,25(OH)2D effectively differentiates PLB-985 cells along a monocytic pathway, providing a useful model of macrophage differentiation.
KW - Macrophages
KW - Respiratory burst
KW - S1 nuclease protection analysis
UR - http://www.scopus.com/inward/record.url?scp=0025946105&partnerID=8YFLogxK
U2 - 10.1002/jlb.50.5.427
DO - 10.1002/jlb.50.5.427
M3 - Article
C2 - 1660912
AN - SCOPUS:0025946105
SN - 0741-5400
VL - 50
SP - 427
EP - 433
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 5
ER -