1-Butyryl-Glycerol: A novel angiogenesis factor secreted by differentiating adipocytes

Deborah E. Dobson, Arbetta Kambe, Eric Block, Teresa Dion, Henry Lu, John J. Castellot, Bruce M. Spiegelman

Research output: Contribution to journalArticlepeer-review

106 Scopus citations


Differentiation of adipocytes is accompanied by secretion of molecules stimulating angiogenesis in vivo and endothelial cell growth and motility in vitro. We demonstrate that the angiogenic and motility-stimulating activities secreted by adipocytes are separable from the endothelial cell mitogenic activity by fractionation of adipocyte-conditioned medium. The major differentiation-dependent angiogenic molecule was purified and identified by GCMS as 1-butyryl-glycerol (monobutyrin). Monobutyrin levels increase at least 200-fold during adipocyte differentiation and represent a major fraction of the total angiogenic activity. Synthetic monobutyrin shows the same spectrum of biological activities as the adipocyte-derived factor: stimulation of angiogenesis in vivo and microvascular endothelial cell motility in vitro, with no effect on endothelial cell proliferation. Angiogenesis is stimulated at doses as low as 20 pg when tested in the chick chorioallantoic membrane assay. These results strongly suggest that monobutyrin is a key regulatory molecule in an angiogenic process linked to normal cellular and tissue development.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
Issue number2
StatePublished - Apr 20 1990


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