γ-Interferon increases expression of class III complement genes C2 and factor B in human monocytes and in murine fibroblasts transfected with human C2 and factor B genes

R. C. Strunk, F. S. Cole, D. H. Perlmutter, H. R. Colten

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

γ-Interferon (IFC-γ) is a well characterized lymphokine known to regulate many mononuclear phagocyte functions, including expression of class I and class II major histocompatibility complex genes. The second component of complement (C2) and factor B are major histocompatibility complex class III gene products synthesized in mononuclear phagocytes. Recombinant IFN-γ increases the synthesis of C2 and factor B in primary cultures of human mononuclear phagocytes and in murine fibroblasts transfected with cosmid DNA bearing the human C2 and factor B genes. In both cell types the increases in C2 and factor B protein synthesis were detected at concentrations of IFN-γ less than 1 unit/ml and the regulation of each was pretranslational. The IFN-γ-induced increases in C2 and factor B mRNA did not require new protein synthesis. In primary cultures of human monocytes, the kinetics of induction of C2 and factor B synthesis differed, but in the transfected L-cells the kinetics were similar, suggesting differences in transduction of the IFN-γ signal, transcriptional, and/or post-transcriptional events in the two cell types. The small size of the factor B 5' flanking region, which is bounded by the 3' terminus of the IFN-γ-regulated C2 gene, provides a well defined region to probe the structural basis for IFN-γ regulation of gene expression.

Original languageEnglish
Pages (from-to)15280-15285
Number of pages6
JournalJournal of Biological Chemistry
Volume260
Issue number28
StatePublished - Dec 1 1985
Externally publishedYes

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