β-Phenylpyruvate and Glucose Uptake in Isolated Mouse Soleus Muscle and Cultured C2C12 Muscle Cells

Ron Ben-Abraham, Vered Gazit, Oded Vofsi, Izahar Ben-Shlomo, Abraham Z. Reznick, Yeshayahu Katz

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Previous investigation demonstrated the potential of β-phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with β-phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM β-phenylpyruvate in soleus muscle (P < 0.01) and C2C12 cells (P < 0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in Vmax compared with controls (P < 0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to β -phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of β-phenylpyruvate.

Original languageEnglish
Pages (from-to)957-963
Number of pages7
JournalJournal of cellular biochemistry
Volume90
Issue number5
DOIs
StatePublished - Dec 1 2003

Keywords

  • Cultured cells
  • Glucose
  • Glucose transporters
  • Phenylketonurias
  • Phenylpyruvic acid
  • Skeletal muscle

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